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INHIBITORY ACTIVITY OF COBALT(II)–MORIN COMPLEX AGAINST THE REPLICATION OF DENGUE VIRUS TYPE 2 Sucipto, Teguh Hari; Churrotin, Siti; Setyawati, Harsasi Setyawati; Mulyatno, Kris Cahyo; Amarullah, Ilham Harlan; Ueda, Shuhai; Kotaki, Tomohiro; Sumarsih, Sri; Wardhani, Puspa; Bendryman, Sri Subekti; Aryati, Aryati; Soegijanto, Soegeng; Kameoka, Masanori
Indonesian Journal of Tropical and Infectious Disease Vol 6, No 6 (2017)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.971 KB) | DOI: 10.20473/ijtid.v6i6.6126

Abstract

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. Antidengue treatments are urgently required to control the emergence of dengue. DENV is a mosquito-borne disease responsible for acute systemic diseases and serious health conditions. DENVs were distributed in the tropical and sub-tropical areas and transmitted to humans by Aedes agypty and Aedes albopictus. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is an important alternative for the characterization and development of drugs. Metal–organic compounds were reported to exhibit fungicidal, bactericidal, and antiviral activities its inhibitory activity was not significant, at high concentration it was more toxic to replicating cells than to stationary cell monolayers of Vero cells. The aim of this study is to investigate the antiviral effects of Cobalt(II)–Morin complex. This compound was further investigated for its inhibitory effect on the replication of DENV-2 in Vero cells. The replication of DENV was measured by enzyme-linked immunosorbent assay and the value of selectivity index (SI). SI was determined as the ratio of the 50% cytotoxic concentration (CC50) to the 50% inhibitory concentration (IC50). The IC50 value of the Cobalt(II)–Morin complex for DENV-2 was 3.08 µg/ml, and the CC50 value of the complex for Vero cells was 3.36 µg/ml; thus, the SI value was 1.09. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of Cobalt(II)–Morin complex and its high toxicity in Vero cells. Further studies are not required before Co(II)–Morin can be applied in the treatment of DENV-2 infections.
PLASMA LEAKAGE PROFILES OF DENGUE HEMORRHAGIC FEVER PATIENTS IN RSUD Dr. SOETOMO, SURABAYA, EAST JAVA, INDONESIA JANUARY – JUNE 2014 Rizaliansyah, Ferdian; Aryati, Aryati; Rusli, Musofa
Indonesian Journal of Tropical and Infectious Disease Vol 6, No 4 (2017)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.998 KB) | DOI: 10.20473/ijtid.v6i4.3456

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Plasma leakage is one crucial point of dengue hemorrhagic fever (DHF) that differentiates it from dengue fever (DF). DHF has to meet 4 criteria which are 2 – 7 days of acute fever, hemorrhagic manifestation, thrombocytopenia (≤100.000 cells/mm3) and evidence of plasma leakage. Plasma leakage consists of increasing hematocrit ≥20%, hypoalbuminemia or evidence of pleural effusion or ascites. Often doctors only base their DHF diagnosis on the presence of thrombocytopenia. This study analyzed the presence of plasma leakage between adult and pediatric patients with a DHF diagnosis in RSUD Dr. Soetomo in order to make the diagnosis and healthcare services better in the future. This was a retrospective study which used medical records of DHF patients admitted from January to June 2014. 78 cases were included, 24 adult patients (31%) and 54 pediatric patients (69%). 29/78 (37%) patients had no evidence of plasma leakage. No adult patients had ascites whereas 11/54 (20%) pediatric patients presented with ascites. No adult patients had pleural effusion whereas 25/54 (53%) pediatric patients did. Most adult patients that had serum albumin checked had normal albumin levels (12/14 [86%]) while only 14/28 (52%) pediatric patients had normal albumin level. 5/22 (23%) adult patients versus 32/53 (60%) pediatric patients showed hematocrit increments ≥20%. Patients admitted with dengue virus infection may currently be often misclassified as DHF because there are no plasma leakage manifestation in some patients.. There are significant differences in plasma leakage manifestations between adult and pediatric patients which poses a theory that pediatric patients are more susceptible to have plasma leakage manifestations than adult patients.
RNA Isolation of Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol. Untoro, Yovilianda Maulitiva; Sucipto, Teguh Hari; Setyawati, Harsasi; Churrotin, Siti; Amarullah, Ilham Harlan; Wardhani, Puspa; Aryati, Aryati; Ueda, Shuhai; Soegijanto, Soegeng
Jurnal Kimia Riset Vol 3, No 1 (2018): Juni
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.739 KB) | DOI: 10.20473/jkr.v3i1.7455

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Dengue virus distributed in tropical and subtropical regions in the world. DENV viruses are transmitted between humans primarily by Aedes aegypti and Aedes albopictus mosquitoes and are endemic in most areas in which the vectors occur. Four serotypes of dengue virus are DENV-1, DENV-2, DENV-3 and DENV-4. DENV-2 is comprised of six genotypes. Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. RNA isolation by combining Guanidinium thiocyanate and phenol reported has been reported. In this report, we investigated RNA isolation from DENV-2 using QIAamp Mini Kit with 2-Isopropanol, Methanol, Chloroform precipitation solvent. Electrophoregram showed DNA band as  the result of RNA isolation with methanol and 2-isopropanol are produced quite well. Dna band of the of RNA isolation with chloroform solvent has the lowest intensity than methanol and 2-isopropanol. This study showed that methanol and 2-isopropanol  can used as precipitation solvent for isolating RNA.
THE RELATIONSHIP OF NS1 ANTIGEN PROFI LE AND DAYS OF ILLNESS IN CHILDREN WITH DENGUE VIRUS INFECTION Puspitasari, Dwiyanti; Dewi, Saraswati; Aryati, Aryati
Jurnal Ners Vol 8, No 1 (2013): April 2013
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.654 KB) | DOI: 10.20473/jn.v8i1.3867

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Introduction: Dengue NS1 Antigen (NS1 Ag) detection or quantification has become a specific diagnostic tool for dengue virus infection, but has variable sensitivity. Previous research reported NS1 Ag level can be detected up to the 7th?10th day of fever, others stated the sensitivity was decreased after four days of fever. We performed this research to analyze the NS1 Ag profile and its relationship with the day of illness.Methods: This study was a prospective cohort study on 39 children hospitalized at Dr. Soetomo Hospital Surabaya since November 2009 to May 2010. Inclusion criteria were acute fever less than 72 hours, bleeding tendency denoted at least by positive tourniquet test, age between 1?14 years, and confirmed by positive dengue IgM/IgG on the 5th day of fever. We performed daily quantitative dengue NS1 Ag tests until defervescence day, and its relationship with the day of illness were analyzed.Results: NS1 Ag was positive in 19/39 samples. Secondary infection occurred in 14/19 of Dengue Fever and 17/20 of Dengue Hemorrhagic Fever cases. Positivity of NS1Ag was higher in primary (6/8 cases) than secondary infection (12/31 cases). Mean level of NS1 Ag was highest on the 2nd day, decreased afterwards and undetected on the 5th day of fever. There was significant relationship between NS1 Ag positivity (P=0.037, rs=-0.9) and level (P<0.001, rs=-1) with the day of illness.Conclusion: Dengue NS1 Ag positivity and level were highest during the acute phase of fever and decreased afterwards.The Relationship of Ns1 Antigen Profi Le and Days of Illness in Children with Dengue Virus Infection
Diagnostic Value of IGFBP-1 Rapid Test and Combined IGFBP-1-AFP in Vaginal Fluid from Premature Rupture of Amniotic Membranes Aryati, Aryati; Kusumawati, Lulut; Sulistyono, Agus
Indonesian Journal of Obstetrics and Gynecology Volume. 3, No. 3, July 2015
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.265 KB) | DOI: 10.32771/inajog.v3i3.42

Abstract

Objective: To compare the diagnostic value of IGFBP-1 and combined IGFBP-1-AFP rapid tests in diagnosing premature rupture of membranes (PROM). Method: This study was conducted in Dr. Soetomo Hospital in Surabaya from July to November 2013. The subjects were 52 pregnant women with presumed PROM diagnosis, which was recorded by clinical data and sampling of vaginal discharge swab. The diagnostic value was obtained by comparing the results of IGFBP-1 and combinated IGFBP-1-AFP rapid tests by standard PROM examination namely vaginal pooling, litmus paper test and ferning test. Result: A difference between the diagnostic value of IGFBP-1 and combined IGFBP-1-AFP rapid tests in diagnosing PROM was shown, where the sensitivity and specificity of IGFBP-1 rapid test was 85% and 95%, compared to combined IGFBP-1-AFP rapid test, which was 91% and 95%. The correlation coefficient of combined IGFBP-1-AFP rapid test with standard PROM examination (r=0.841, p=0.000) was higher than the correlation coefficient of IGFBP-1-AFP rapid test with standard PROM examination (r=0.772, p=0.000). Conclusion: Combined IGFBP-1-AFP rapid test has a better diagnostic value than IGFBP-1 rapid test alone. Keywords: combined IGFBP-1-AFP, IGFBP-1, PROM
NILAI DIAGNOSTIK RAPID TEST TBAG DAN MPT64 DENGAN KULTUR SEBAGAI GOLD STANDARD Nazarudin, Muhammad; Nugraha, Jusak; Aryati, Aryati
Jurnal Biosains Pascasarjana Vol 17, No 3 (2015): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (552.873 KB) | DOI: 10.20473/jbp.v17i3.2015.141-155

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AbstrakTuberkulosis (Tb) sampai kini masih dianggap sebagai salah satu penyakit berbahaya yang ada di muka bumi. Pada tahun 2012, diperkirakan 8,6 juta orang mengidap Tb dan 1,3 juta di antaranya meninggal dunia. Mycobacterium tuberculosis sebagai penyebab penyakit Tb, dapat dideteksi dengan pengecatan BTA untuk mewarnai bagian sitoplasma dari bakteri ini. Standar terbaik dalam pemeriksaan Tb adalah kultur bakteri, namun dinilai terlalu lama dalam memberikan hasil. Beberapa produsen mengembangkan alat yang mempermudah prosedur pemeriksaan, memberikan hasil yang cepat namun tetap berkualitas, diantaranya yang beredar di Indonesia adalah TbAg Rapid Test dan MPT64 Rapid Test. Metode penelitian ini adalah analitik observasional dengan rancangan cross sectional. Dilakukan terhadap 50 responden dengan 34 responden mengidap Tb paru. Data yang diuji nilai diagnostik, uji McNemar dan uji Kappa. Nilai sensitivitas, TbAg Rapid Test dengan sampel sputum 97.5% dan isolat 50%. Nilai sensitivitas MPT64 Rapid Test dengan sampel sputum 11.76% dan isolat 95%. Hasil BTA dan TbAg Rapid Test juga tidak menunjukkan perbedaan berarti sehingga dapat digunakan untuk pemeriksaan skrining dan MPT65 Rapid Test dapat gunakan untuk tes biokimia setelah kultur. Kata Kunci : Tuberkulosis, TbAg Rapid Test, MPT64 Rapid Test
RNA ISOLATION OF DENGUE VIRUS TYPE 1 WITH DIFFERENT PRECIPITATION SOLVENTS: DIMETHYL SULFOXIDE, ACETONE, AND ETHANOL 70% Maharani, Anisa; Sucipto, Teguh Hari; Setyawati, Harsasi; Churrotin, Siti; Amarullah, Ilham Harlan; Wardhani, Puspa; Aryati, Aryati; Ueda, Shuhai; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 3 (2018)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.948 KB) | DOI: 10.20473/ijtid.v7i3.6748

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Dengue Hemorrhagic Fever (DHF) is caused by dengue viruses that belong to Flaviviridae. The disease is known to be caused by 4 types of dengue viruses, namely DENV-1, DENV-2, DENV-3, and DENV-4 associated with antigenic. Dengue virus is a virus RNA that causes illness with clinical manifestations of Dengue Fever, Dengue Hemorrhagic Fever and Dengue Shock Syndrome. The aim of research was to determine the effectiveness of dimethyl sulfoxide, acetone, and ethanol 70% as precipitation solvent in the process of RNA isolation. The method used was Reverse Transcription - Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) with specific primers for dengue virus type 1 (DENV-1). RNA isolation can be done easily using an RNA Isolation Kit. Use of RNA Isolation Kit results in a purer RNA isolate from contaminants and from RNA degradation. In generally the isolation is using cold ethanol / alcohol with concentration 90-95%. Ethanol / Alcohol does not dissolve RNA and light density of alcohol lighter than water makes RNA rise and hover on the surface. In RNA isolation solvent precipitation that used are acetone, ethanol 70%, and DMSO. In qualitative RNA measurements using agarose gel electrophoresis and was examined under the UV light-illuminator and quantitative RNA measurements using Nanodrop spectrophotometry with absorbance ratio at 260/280 and 260/230 showed a good result indicated by the appearance of the band on electrophoresis results in PCR. While the measurement quantitatively is showed that there was still protein contamination but the results are quite good because it does not much different from the ratio set in the reference. Acetone, ethanol 70%, and DMSO can be used as a substitute of 96% ethanol in the process of RNA isolation in DENV-1 virus and can also be applied to other dengue virus because the structure of the 4th antigen serotype is very similar one with the other and no effect.
Precipitation Solvents for RNA Extraction of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, and Ultrapure H2O Putri, Rizqidhana Juliana; Sucipto, Teguh Hari; Setyawati, Harsasi; Churrotin, Siti; Amarullah, Ilham Harlan; Wardhani, Puspa; Aryati, Aryati; Soegijanto, Soegeng
Jurnal Kimia Riset Vol 3, No 2 (2018): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477.838 KB) | DOI: 10.20473/jkr.v3i2.9353

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Dengue is a disease caused by a virus from the family Flaviviradae, carried by a female mosquito of Aedes aegypti species. Dengue fever is widespread in the tropic areas. It caused by rainfall, temperature and unplanned urbanization. According to the ministry of health , almost all provinces in Indonesia are endemic areas of dengue fever. In 2014, up to mid-December Dengue Hemorrhagic Fever (DHF) patients in 34 provinces in Indonesia are 71,668 people and 641. This figure is lower than the previous year, 2013 with 112,511 people and 871 deaths . This disease consists of four types of serotypes, namely DENV-1, DENV-2, DENV-3, and DENV-4. This disease can be identified using a variety of methods, one of the method is Reverse Transcription - Polymerase Chain Reaction (RT-PCR) method. This study aims to determine the ability of Dimethylformamide (DMF), Ethylenediamintetraacetic Acid (EDTA), and Ultrapure H2O as the substitute of  Ethanol for precipitation in RNA extraction process. The sample used in this research obtained from Surabaya. RNA extraction itself can be done by using a special kit for RNA extraction. In Reverse Transcription - Polymerase Chain Reaction method, first RNA is extracted and then transcribed back (Reverse Transcription) which then form cDNA that later will be amplified by using PCR method. In this study used specific primers for dengue virus type 3 (DENV-3). The results of this study show that DMF, EDTA, and Ultrapure H2O can be used as the substitute of Ethanol for precipitation on RNA extraction. The result is evidenced by the formation of viral DNA bands on gel electrophoresis results.
ANALISIS PERBANDINGAN KINERJA REKSADANA SAHAM SYARIAH DENGAN REKSADANA SAHAM KONVENSIONAL DI INDONESIA Lianti, Lianti; Aryati, Aryati; Ramaya, Nurul
Ekonis: Jurnal Ekonomi dan Bisnis Vol 18, No 2 (2017): JURNAL EKONOMI DAN BISNIS (EKONIS)
Publisher : Ekonis: Jurnal Ekonomi dan Bisnis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.886 KB) | DOI: 10.30811/.v18i2.402

Abstract

Abstrak : Penelitian ini bertujuan untuk mengetahui perbandingan antara kinerja reksadana saham syariah dengan reksadana saham konvensional di Indonesia. Populasi penelitian ini adalah seluruh reksadana saham syariah dan reksadana saham konvensional yang terdaftar di Otoritas Jasa Keuangan selama periode 1 Januari 2014 s.d. 31 Desember 2016. Pemilihan sampel dilakukan dengan menggunakan metode purposive sampling. Jumlah sampel yang terpilih adalah sebanyak 27 sampel yang terdiri dari 8 reksadana saham syariah dan 19 reksadana saham konvensional. Teknik yang digunakan untuk menilai kinerja kedua jenis reksadana saham adalah Indeks Sharpe dan Indeks Treynor. Pengujian hipotesis dengan menggunakan Independent Sample T-Test pada α sebesar 5% dan merupakan uji dua sisi dengan digunakan α/2 sebagai pembanding yaitu 2,5%. Hasil penelitian ini menyimpulkan bahwa secara rata-rata kinerja reksadana saham konvensional sedikit lebih baik jika dibandingkan dengan reksadana saham syariah. Akan tetapi, perbedaan kinerja diantara reksadana saham syariah dan reksadana saham konvensionalyang diteliti tidak terdapat perbedaan secara signifikan baik dengan Indeks Sharpe maupun Indeks Treynor. Kata Kunci: Reksadana Saham, Kinerja , Syariah, dan Konvensional
Diagnostic Value of IGFBP-1 Rapid Test and Combined IGFBP-1-AFP in Vaginal Fluid from Premature Rupture of Amniotic Membranes Aryati, Aryati; Kusumawati, Lulut; Sulistyono, Agus
Indonesian Journal of Obstetrics and Gynecology Volume. 3, No. 3, July 2015
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.265 KB) | DOI: 10.32771/inajog.v3i3.42

Abstract

Objective: To compare the diagnostic value of IGFBP-1 and combined IGFBP-1-AFP rapid tests in diagnosing premature rupture of membranes (PROM). Method: This study was conducted in Dr. Soetomo Hospital in Surabaya from July to November 2013. The subjects were 52 pregnant women with presumed PROM diagnosis, which was recorded by clinical data and sampling of vaginal discharge swab. The diagnostic value was obtained by comparing the results of IGFBP-1 and combinated IGFBP-1-AFP rapid tests by standard PROM examination namely vaginal pooling, litmus paper test and ferning test. Result: A difference between the diagnostic value of IGFBP-1 and combined IGFBP-1-AFP rapid tests in diagnosing PROM was shown, where the sensitivity and specificity of IGFBP-1 rapid test was 85% and 95%, compared to combined IGFBP-1-AFP rapid test, which was 91% and 95%. The correlation coefficient of combined IGFBP-1-AFP rapid test with standard PROM examination (r=0.841, p=0.000) was higher than the correlation coefficient of IGFBP-1-AFP rapid test with standard PROM examination (r=0.772, p=0.000). Conclusion: Combined IGFBP-1-AFP rapid test has a better diagnostic value than IGFBP-1 rapid test alone. Keywords: combined IGFBP-1-AFP, IGFBP-1, PROM
Co-Authors Achmad Noerkhaerin Putra, Achmad Noerkhaerin Adissadah, Avina Fimas Agus Santosa, Agus Agus Sulistyono Agustin Iskandar Aksono H, Eduardus Bimo Akualing, Jeine Stela Akualing, Jeine Stela Amarullah, Ilham Harlan Amarullah, Ilham Harlan Amarullah, Ilham Harlan Ambar, Nabil Salim Andriani, Suci Anniwati, Leonita Arfijanto, M Vitanata Arifah, Budi Bastiana Bastiana betty Agustina Tambunan, betty Agustina Butarbutar, Trieva Verawaty Darto Saharso Dewata, Desak Gde Ushadi Bulan Dewi, Pande Putu Ayu Patria Doddy M. Soebadi Dominicus Husada, Dominicus Dwi Rahayuningsih Dwiyanti Puspitasari, Dwiyanti Eko Sulistijono Ekowati, Lestari Erawati, Erawati Erni Juwita Nelwan, Erni Juwita Erwin Astha Triyono, Erwin Astha Fahimah Martak Fuadi, M. Robi?ul Gondo Mastutik, Gondo Habsari, Janti Tri Hajat, Arifoel Hajat, Arifoel Hardiono Hardiono, Hardiono Harianto Notopuro Harsasi Setyawati, Harsasi Hartono, Pudjo Henny Elfira Yanti, Henny Elfira Heny Arwati, Heny Hermina Novida, Hermina Ire Puspa Wardhani Ismaillya Noor, Roudhotul Johanis, Johanis Jusak Nugraha Karimah, Azimatul Kartika Sari, Sri Kris Cahyo Mulyatno, Kris Cahyo Kuntaman Kuntaman Kurniasari, Diyan Wahyu Kusmiati, Tutik Kusmiati, Tutik LIANTI, LIANTI Lulut Kusumawati M Viany S, Carolina M. Andriady S. Nasution Maharani, Anisa Manu, Thomas Tandi Marhana, Isnin Anang Marpaung, Ferdy Royland Marpaung, Ferdy Royland Marsudi, Djoko Mas Bayu Syamsunarno, Mas Bayu Masanori Kameoka, Masanori Masyeni, Sri Maula, Irhas Malik Mufasirin Mufasirin, Mufasirin Muhammad Nazarudin, Muhammad Muhashonah, Izzuki Mustahal Noor, Roudhotul Ismaillya Nurdianto, Arif Rahman Pawarti, Dwi Reno Pawarti, Dwi Reno Pindayani, Kusuma Pranidya, Nada Putri Prihatini Prihatini, Prihatini Probohoesodo, M. Y. Probohoesodo, M.Y. Probohoesodo, M.Y. Probohoesodo, Y. Purnomo, Windu Puspita Rini, Diah Puspitasari, Yessy Putri Wirawati, I A Putri, Rizqidhana Juliana Rachmayanti, Notrisia Rahmadhona, Devi Ramadhani, M Thohirin Ramaya, Nurul Ranoko, Merylin Resna, Resna Retno Setyowati, Ety Rizaliansyah, Ferdian Rochaeni, Ade Rochaeni, Ade Rusli, Musofa Saraswati Dewi, Saraswati Sari, Sri Kartika Sasmono, R. Tedjo Semedi, Bambang Pujo Setyawati, Harsasi Setyawati Simangunsong, Adil Dinata Siti Churrotin, Siti Soegeng Soegijanto Soemarsono, Juli Sofro, Muchlis AU Sri Subekti Bendryman, Sri Subekti Sri Sumarsih Suhintam Pusarawati Sunaryo Hardjowijoto Suryokusumo, Mohammad Guritno Suwasanti, Niluh Teguh Hari Sucipto, Teguh Hari Tomohiro Kotaki, Tomohiro Trihabsari, Janti Triyono, Erwin Ueda, Shuhai Ueda, Shuhai Untoro, Yovilianda Maulitiva Usman Hadi Wibrianto, Aswandi Widajati, Rahma Widodo J Pujiraharjo Windu Nafika Wiradewi Lestari, A A Yohan, Benediktus Yulia Iriani, Yulia