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Quality of Epididymal and Ejaculated Sperms of Spotted Buffalo in Dextrose Supplemented Extender Yulnawati, .; Gunawan, Muhammad; Maheshwari, Hera; Rizal, Muhammad; Herdis, .; Boediono, Arief
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (32.619 KB) | DOI: 10.4308/hjb.17.1.27

Abstract

Quality of epididymal and ejaculated sperms of spotted buffalo in three different extenders (i.e. Andromed, Andromed supplemented with 0.2% dextrose, and Andromed supplemented with 0.4% dextrose) was studied.  The results showed that there was no significantly different (P > 0.05) quality of ejaculated and epididymal sperms.  The motility percentage of post thawing epididymal and ejaculated sperms was 41-46% and 41-45%, respectively.  And percentage of cytoplasmic membrane integrity of the post thawing epididymal and ejaculated sperms was 66-67% and 47-54% respectively. There was also no significant different (P > 0.05) in the percentage of cytoplasmic membrane integrity between the extenders of both ejaculated and epididymal sperms. This result suggested that epididymal sperms were reliable for artificial insemination as good as ejaculated sperms.
UJI EFEKTIVITAS ANTIHIPERURISEMIA EKSTRAK AIR DAUN SALAM (Syzygium polyanthum Wight.) TERHADAP MENCIT JANTAN (Mus musculus) YANG DIINDUKSI JUS HATI AYAM DAN KALIUM OKSONAT Hidayah, Nurul; Hasanah, Fenny; Gunawan, Muhammad; Lestari, Anggun
JURNAL PENELITIAN SAINTIKA Vol 18, No 1 (2018): MARET 2018
Publisher : JURNAL PENELITIAN SAINTIKA

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Abstract

Hiperurisemia disebabkan oleh sintesa purin berlebihan dalam tubuh karena pola makan yang tidak sehat dan proses pengeluaran asam urat dari dalam tubuh yang mengalami gangguan. Penggunaan obat-obat sintesis untuk hiperurisemia bukan tanpa risiko efek samping yang merugikan, sehingga diperlukan terapi alternative dari alam. Daun salam dipercaya dapat menurunkan kadar asam urat. Tujuan dari penelitian ini adalah untuk mengetahui potensi efektivitas ekstrak air daun salam terhadap penurunan kadar asam urat mencit jantan yang diinduksi dengan jus hati ayam 0,2% dan kalium oksonat 250 mg/kg BB. Tahapan penelitian meliputi identifikasi tanaman, pembuatan simplisia dan ekstrak, standarisasi dan skrining fitokimia, serta pengujian efek antihiperurisemia. Hewan uji yang digunakan sebanyak 24 ekor, yang dibagi menjadi 6 kelompok uji. Pembagian kelompok hewan uji terdiri atas kelompok normal, induksi, pembanding, ekstrak air daun salam (EADS) dosis 50 mg/kg BB, 100 mg/kg BB, dan 200 mg/kg BB. Parameter efek antihiperurisemia yang diamati adalah kadar asam urat darah hewan uji. Data yang diperoleh selanjutnya diolah secara statistik dengan interval confidency 95%. Hasil uji skrining fitokimia menunjukkan bahwa simplisia dan EADS mengandung tannin, flavonoid, saponin, glikosida, dan alkaloid. Semua kelompok dosis EADS menunjukkan potensi antihiperurisemia yang tidak berbeda signifikan dengan kelompok pembanding yang digunakan.
Pemetaan Psikografis Kependudukan Untuk Kepentingan Kampanye Pilkada (Studi Kasus: Kota Cimahi) Gunawan, Muhammad; Sumarno, -; Indrianawati, -
REKA GEOMATIKA Vol 2016, No 2 (2016)
Publisher : Institut Teknologi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1493.523 KB) | DOI: 10.26760/jrg.v2016i2.1853

Abstract

ABSTRAKPemilihan kepala daerah (pilkada) memberikan kesempatan kepada masyarakat untuk memilih kepala daerahnya tanpa melalui suatu perwakilan. Dalam pelaksanaan pilkada terdapat proses kampanye yang bertujuan meyakinkan pemilih untuk memilih calon pasangan tertentu. Tujuan dari pemetaan psikografis kependudukan untuk kepentingan kampanye adalah memberikan data awal kampanye dan lokasi potensial kampanye. Penelitian ini menggunakan metode analisis statistika spasial dengan metode Global Moran’s I dan Getis Ord-­G terhadap data karakteristik kependudukan untuk mengetahui adanya autokorelasi spasial dan bentuk pola kluster antara unit wilayah (kelurahan) di Kota Cimahi. Berdasarkan hasil analisis statistika spasial, dapat diketahui bahwa di seluruh kelurahan Kota Cimahi, data awal kampanye yang relevan adalah data karakteristik pekerjaan dan agama. Karakteristik pendidikan relevan di seluruh kelurahan Kota Cimahi, kecuali Kelurahan Cimahi dan Karangmekar. Karakteristik usia juga relevan di seluruh kelurahan Kota Cimahi, kecuali Kelurahan Cimahi dan Pasirkaliki.Kata kunci: kampanye, statistika spasial, autokorelasi, pola klusterABSTRACTRegional heads election (Pilkada) has given an opportunity to people for choosing their regional heads without any delegation in general election. The general election includes campaign process, which aims to select a certain candidate. In the campaign process, psychographic mapping of demography aims to give preliminary data and potential location of the campaign. This research has used spatial statistical analysis using Global Moran's I and Getis Ord-­G methods for analysing demographic characteristics. The methods aim to know spatial autocorrelation as well as cluster pattern between villages in Cimahi District. The analysis result shows the relevant characteristics in all Cimahi villages are job and religion. Meanwhile, education characteristic is relevant in all villages Cimahi, except Cimahi and Karangmekar villages. Age characteristic is also relevant in all villages Cimahi, except Cimahi and Pasirkaliki villages.Keywords: campaign, spatial statistic, autocorelation, cluster pattern
Optimalisasi Perancangan Jaringan 4G LTE Pada Frekuensi 2300 MHZ Berbasis TDD Di Kabupaten Rokan Hulu Gunawan, Muhammad; Sari, Linna Oktaviana
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Long Term Evolution (LTE) is a technology developed by 3GPP as a development for cellular communication technology. LTE is a technology that can send and receive data at high speed. In Rokan Hulu Regency there are several areas that have not been reached by LTE and have a low Reference Signal Receipt Power (RSRP) value of approximately -100 dBm from ≥ -95 dBm from the standard. This study aims to optimize the LTE network based on planning by coverage and planning by capacity using the TDD radio access method. Specified frequency LTE is 2300 MHz, it is using 5 MHz and 10 MHz bandwidth. Coverage based on signal levels with a frequency of 2300 MHz with non-contoured maps at 5 MHz and 10 MHz bandwidth has a signal level value of ≥ -95 dBm at 99.99% and 100%. Coverage of signal level simulation results with contoured maps at 5 MHz and 10 MHz bandwidth has a signal level value of ≥ -95 dBm at 71.2% and 76.43%. So based on these parameters it can be concluded that the TDD technique with a bandwidth of 10 MHz is feasible to apply to Rokan Hulu Regency.Keywords: LTE, planning by coverage, planning by capacity, TDD, bandwitdh, signal level.
KEWENANGAN DEWAN PEMBINA DAN PENGENDALI PENGELOLAAN KOLABOATIF (DP3K) TAMAN NASIONAL KAYAN MENTARANG (TNKM) NASIONAL KAYAN MENTARANG (TNKM) Gunawan, Muhammad
JURNAL AKTA YUDISIA Vol 2, No 1 (2017): Jurnal Akta Yudisia Vol. 2 Nomor 1
Publisher : Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.633 KB) | DOI: 10.35334/ay.v2i1.977

Abstract

Abstract KMNP (Taman Nasional Kayan Mentarang) collaborative management essentially an innovation in the management of National Parks in Indonesia that promote mutual interests, shared responsibility, the role and benefits between government, local communities and NGOs. In this paper, the issue raised is the authority of the Dewan Pembina dan Pengendali Pengelolaan Kolaboratif (DP3K) KMNP. The aim of this study was to determine and analyze the authority of the Board of Trustees and Collaborative Management Controller (DP3K) Kayan. This type of research is the study of normative juridical approach to legislation and conceptual approach and the use of primary and secondary law with a qualitative analysis approach. Based on the results, the conclusion that the authority DP3K TNKM among others embodies the aspirations of the parties in order to develop and control the implementation of collaborative management KMNP.Keywords: National Parks, collaborative management, Authority.
RASIO HUBUNGAN NILAI DAYA DUKUNG TIANG PANCANG BERDASARKAN PENGUJIAN SONDIR, KALENDERING DAN TES PDA PADA JEMBATAN PELAWA KABUPATEN PARIGI MOUTONG Gunawan, Muhammad; Oktaviana, Ida Sri; B., Arifin
JOURNAL TEKNIK SIPIL DAN INFRASTRUKTUR Vol 4, No 1 (2014)
Publisher : Jurusan Teknik Sipil Universitas Tadulako

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Abstract

Bearing capacity of pile foundation bearing capacity is influenced by several factors, such as the amount of applied loads and non-homogeneous soil characteristics. To determine the bearing capacity of single pile foundation the static method is used on the basis of CPT data and dynamic methods based on dynamic pile and PDA tests. Pile foundations of the Pelawa bridge used in this study are located in the District of Parigi - Moutong. The study of literatures and secondary data such as the data of driven pile equipment, driven piles , CPT, dynamic pile tests, and PDA tests are used for analyzing the pile bearing capacity . Meyerhof method, Aoki de Alencar, LCPC, Price and Waldre, and Schmertmann are used to determine the bearing capacity of pile based on sondir (CPT) data. The dynamic pile tests data are used to calculate the bearing capacity of the piles by using methods of Hiley, Olsen and Flaate , Enginering News Record (ENR) , Janbu Mansur and Hunter , and AASHTO . The results show that  the lowest dan the highest of the ultimate bearing capacities  for static methods (CPT data) are  114.096 tons (Aoki De Alencar) and 305.479 tons (Schmertmann) respectively. Kalendering method (dynamic pile data) show that the lowest value of 284.22 tons were obtained from equation given by Hiley and the highest 1450.26 tons were obtained from equation given by  Janbu.The range of 171.2 tons - 174.5 tons were given for PDA test data. The ratio between CPT to PDAs results that are closest to the value of 1 which is 0.938 is the value that obtained from Meyerhof’s equation, while the ratio between dynamic method (kalendering) to PDAs results that are closest to the value of 1 which is 1,660 is the value that obtained from Hiley equation Keywords: single pile bearing capacity, CPT, dynamic pile test, PDA test
UJI EFEKTIVITAS FLAVONOID TOTAL EKSTRAK ETANOL KULIT BATANG NANGKA PENETAPAN KADAR FOSFOR DALAM OKRA HIJAU (Abelmoschus esculentus (L.) Moench) SECARA SPEKTROFOTOMETRI SINAR TAMPAK Saputri, Muharni; Gunawan, Muhammad; Maghfirah, Maizatun
Journal of Pharmaceutical And Sciences Vol 1 No 1 (2018): JPS Volume 1 Nomor 1 (2018)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v1i1.6

Abstract

Green Okra is one vegetable that contains minerals is quite high. One such mineral is phosphorus, which is an essential mineral needed for growth and repair of cells in the body. This study aims to determine the levels of phosphorus in raw green okra, steamed, and stew as commonly consumed by people. The research method using wet digestion analysis and measurement with visible spectrophotometry with the addition of phosphorus reagent color developer which is a mixture ammonium molibdat 4%, ascorbic acid 0.1 N, antimonil potassium tartrate, and 5N sulfuric acid produces a blue color and is measured at a wavelength of 722, 50 nm with the working time in the 30th minute to the 38th minute. Levels of phosphorus obtained from raw green okra 62.115 mg / 100 g, steamer 60.130 mg / 100 g,
DEVELOPMENT OF MICE EMBRYO (MUS MUSCULUS L.) AFTER CLOSED PULLED STRAW VITRIFICATION IN CZB MEDIUM Gunawan, Muhammad; Kaiin, Ekayanti Mulyawati; Rusherdiannita, Raden Cindy; Alipin, Kartiawati
ANNALES BOGORIENSES Vol 23, No 2 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (952.946 KB) | DOI: 10.14203/ann.bogor.2019.v23.n2.72-80

Abstract

The objective of our present experiment was to investigate the effects of closed pulled straw (CPS) vitrification on the viability and development of mouse embryo. The experiment was arranged according to completely randomized design (CRD) consisting of 4 treatments, namely not vitrification (NV) is control, CPS 1 (mCZB Hepes + 20% Bovine Serum Albumin (BSA) +0.5 M sucrose + 10% EG + 10% DMSO), CPS 2 (mCZB Hepes + 20% BSA + 0.5 M sucrose + 15% EG + 15% DMSO), and CPS 3 (mCZB Hepes + 20% BSA +0.5 M sucrose + 20% EG + 20% DMSO) with 6 replications. The viability of embryos (%) was determined after 24 - 72 h of the culture period, while we also observed the percentage of embryos reaching the blastocyst stage (early blastocyst, expanded, hatching, and hatched). As a result, the treatments did not give a significant difference in the viability of embryos (P<0,05) but showed significant effects on embryo development (P<0,05). Furthermore, this present work conclusively found that CPS vitrification in CZB medium with cryoprotectants ethylene glycol (EG) and dimethyl sulfoxide (DMSO) noticeably influenced the development of mice embryo to reach the blastocyst stage, but showed no remarkable difference in the viability of embryo after culture for 24 ? 72 h.
KEWENANGAN DEWAN PEMBINA DAN PENGENDALI PENGELOLAAN KOLABOATIF (DP3K) TAMAN NASIONAL KAYAN MENTARANG (TNKM) NASIONAL KAYAN MENTARANG (TNKM) Gunawan, Muhammad
JURNAL AKTA YUDISIA Vol 2, No 1 (2017): Jurnal Akta Yudisia Vol. 2 Nomor 1
Publisher : Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/ay.v2i1.977

Abstract

Abstract KMNP (Taman Nasional Kayan Mentarang) collaborative management essentially an innovation in the management of National Parks in Indonesia that promote mutual interests, shared responsibility, the role and benefits between government, local communities and NGOs. In this paper, the issue raised is the authority of the Dewan Pembina dan Pengendali Pengelolaan Kolaboratif (DP3K) KMNP. The aim of this study was to determine and analyze the authority of the Board of Trustees and Collaborative Management Controller (DP3K) Kayan. This type of research is the study of normative juridical approach to legislation and conceptual approach and the use of primary and secondary law with a qualitative analysis approach. Based on the results, the conclusion that the authority DP3K TNKM among others embodies the aspirations of the parties in order to develop and control the implementation of collaborative management KMNP.Keywords: National Parks, collaborative management, Authority.
INFLUENCES OF INCUBATION TIME AND SUCROSE CONCENTRATION ON MICE (Mus musculus L.) OOCYTE VIABILITY FOR ENUCLEATING PROCEDURE Kaiin, Ekayanti Mulyawati; Gunawan, Muhammad; Madihah, Madihah; Nafisah, Ghina
Jurnal Kedokteran Hewan Vol. 12 No. 3: September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.10896

Abstract

This study aimed to find out the optimum incubation time to complete mouse oocyte maturation at Metaphase II (MII) stage and determine the optimum sucrose concentration enabling to induce nuclear swelling for visualization that is important for enucleating process at the initial procedure of somatic cell nuclear transfer (SCNT). In this current study, mice were used as animal model. Completely randomized design was arranged, consists of 2 trials with 4 treatments and 7 replications. In the first trial, the oocytes were cultured at 0-2, 4-6, 8-10, and 12-14 h in 5% CO2 incubator at 37 C. Second, the MII oocytes obtained from previous trial were cultured in M199 medium containing different concentrations of sucrose (0, 1.5, 3, and 6%). The parameters measured were the oocyte viability at various stages, i.e germinal vesicle (GV), metaphase I (MI), anaphase/telophase I (A/T I), and metaphase II (MII), and the viability of swollen nuclear oocytes using Hoechst/PI staining. The results showed that the optimum incubation time required by oocytes to reach MII stage was 12-14 h with a percentage of 57.14±12.67%, while the optimum sucrose concentration for nuclear swelling was found at 3% with a percentage of 100±0.00%. Our findings provided preliminary results related to the maturation process of the mouse oocyte nucleus, which is meaningful for the initial procedure of SCNT.