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ISOLASI, IDENTIFIKASI DAN PEMANFAATAN FUNGI YANG BERASOSIASIDENGAN Tristaniopsis obovata Turjaman, Maman; Faulina, Sarah Asih; Aryanto, Aryanto; Najmulah, Najmulah; Yani, Ahmad; Hidayat, Asep
Jurnal Penelitian Hutan dan Konservasi Alam Vol 16, No 1 (2019): Jurnal Penelitian Hutan dan Konservasi Alam
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1447.869 KB) | DOI: 10.20886/jphka.2019.16.1.73-90

Abstract

Sari Tristaniopsis obovata (pelawan) bersimbiosis dengan fungi ektomikoriza yang  menghasilkan tubuh buah fungi yang dapat dikonsumsi (edible mushroom) dan bunga pelawan sebagai sumber nektar untuk lebah hutan yang memproduksi madu pahit yang bernilai ekonomi tinggi. Permasalahan yang dihadapi saat ini adalah berkurangnya luasanhutan kerangas akibat konversi hutan sehingga luasan hutan pelawan menurun. Penelitian ini bertujuan untuk mendapatkan informasi potensi fungi yang berasosiasi dengan Tristaniopsis obovata dan uji pemanfaatan fungi yang berpotensi untuk memacu pertumbuhan bibit T. obovata. Penelitian dilakukan melalui pengumpulan sumber benih/anakan alam, isolasi dan identifikasi molekuler ITS rDNA fungi yang berasosiasidengan T. obovata, perbanyakan anakan melalui stek pucuk, dan inokulasi fungi yang berpotensi memacu pertumbuhan bibit T. obovata. Hasil identifikasi molekuler menunjukkan bahwa diperoleh 3 isolat yang berfungsi sebagai ektomikoriza, yaitu 4PK1 (Corticiaceae 1), 17BK2 (Corticiaceae 2), dan 24PK4 (Cortinarius sp.). Inokulasi fungi ektomikoriza memberikan pengaruh pada pertumbuhan tanaman dan kandungan nutrisi bibit anakan pelawan. Teknik stek pucuk dengan KOFFCO cukup efektif untuk memperbanyak bibit pelawan dengan tingkat keberhasilan sekitar 50%. Keberhasilan pada penelitian ini menjadi dasar IPTEK untuk melestarikan produktivitas ekosistem hutan kerangas.
DECOLORIZATION OF AZO DYES AND MINERALIZATION OF PHENANTHRENE BY TRAMETES SP. AS03 ISOLATED FROM INDONESIAN MANGROVE FOREST Hidayat, Asep; Tachibana, Sanro
Indonesian Journal of Forestry Research Vol 1, No 1 (2014): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Textile industry contributes the most disposals of synthetic dyes, and about 40% of textile dyes has been generating high amount of colored wastewater. Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene, is a group of organic compounds, that structurally comprised of two or more benzene rings, which persist in air, water, and soil. The organic pollutants of dyes and PAHs have adversely effects the food chain and are potentially toxic, mutagenic, and carcinogenic to the environment. The objective of this research is to screen and investigate the potential fungus from mangrove forest to degrade azo dyes and phenanthrene.  In this study, fungi were collected from mangrove forest in Riau Province – Sumatra – Indonesia. Previously, Trametes sp. AS03 is one of the fungi isolated from mangrove forest in Riau Province, that was able to decolorize Remazol Brilliant Blue R (RBBR). The capability of Trametes sp. AS03 to decolorize four azo dyes, Remazol B. Violet (V5), Levafix Orange E3GA (Or64), Levafix B. Red E-6BA (R159), and Sumifix S. Scarlet 2GF (R222), were further evaluated. The result shows that Trametes sp. AS03 decolorized 91, 60, 48, and 31 of V5, R222, R159, and Or64, respectively. By showing its capability to decolorize some of the dyes, Trametes sp. AS03 was used to break down phenanthrene. AS03 degraded more than 70% of phenanthrene in 15 days.
UJI ASAL SUMBER BIBIT NILAM (POGOSTEMON CABLIN BENTH.) DI PASAMAN BARAT SUMATERA BARAT Junaedi, Ahmad; Hidayat, Asep
Jurnal Penelitian Hasil Hutan Vol 28, No 3 (2010): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Penelitian dan Pengembangan Hasil Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3083.564 KB) | DOI: 10.20886/jphh.2010.28.3.241-254

Abstract

Uji asal sumber bibit nilam di Pasaman Barat, Sumatera Barat perlu dilakukan sebagai salah satu tahapan untuk meningkatkan produktivitas hasil panen dan minyak nilam. Penelitian ini dilakukan untuk menguji tiga asal sumber bibit nilam di Pasaman Barat, Sumatera Barat. Rancangan acak kelompok dilakukan pada penelitian dengan menguji tiga asal sumber bibit nilam sebagai perlakuan yaitu bibit nilam asal Tapak Tuan, asal Dairi dan Sidikalang. Hasil penelitian menunjukkan bahwa pertumbuhan, potensi produksi terna kering, potensi produksi minyak nilam dan kulitas minyak terbaik ditunjukkan oleh nilam asal sumber bibit asal Dairi. Pemanenan pada umur sebelas bulan setelah tanam, nilam asal bibit Dairi menghasilkan potensi produksi terna kering 19,4 ton/ha, potensi produksi minyak 208 kg /ha dan kandungan patchouli alkohol 44,57%.
PENGARUH UKURAN DIAMETER STEK BATANG Hopea odorata Roxb. DARI KEBUN PANGKAS TERHADAP KEMAMPUAN BERTUNAS, BERAKAR, DAN DAYA HIDUPNYA Hidayat, Asep; Hendalastuti R, Henti; Nurohman, Edi
Jurnal Penelitian Hutan dan Konservasi Alam Vol 4, No 1 (2007): Jurnal Penelitian Hutan dan Konservasi Alam
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphka.2007.4.1.1-12

Abstract

Penelitian ini bertujuan untuk mendapatkan informasi tentang besarnya   pengaruh kelas diameter sumber bahan stek Hopea odorata Roxb. terhadap kemampuan membentuk tunas, berakar, dan daya hidup stek. Tanaman sumber bahan stek (ortet) dibagi berdasarkan kelas diameter dan masing-masing kelas diameter dianalisis untuk melihat kelas diameter ortet yang paling produktif menghasilkan stek berkualitas. Hasil penelitian menunjukkan bahwa kelas diameter ortet berpengaruh sangat nyata terhadap pembentukan jumlah tunas, panjang tunas, dan bahan stek yang dihasilkan. Kemampuan bertunas tertinggi adalah bahan stek asal kelas diameter ortet IV (0,78-0,94 cm) dan V (0,95-1,11 cm) dengan nilai 63 % dan 60 %. Kemampuan berakar dan daya hidup bahan stek dari semua kelas diameter ortet di atas 90 %.
EFEKTIVITAS BAHAN AJAR AUDIO VISUAL MYOB DALAM PEMBELAJARAN PRAKTIKA KOMPUTER AKUNTANSI Hidayat, Asep
Jurnal Kajian Teknologi Pendidikan Vol 3, No 1 (2020)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1017.937 KB) | DOI: 10.17977/um038v3i12019p097

Abstract

Abstrak: Penelitian ini bertujuan untuk menguji coba secara terbatas efektivitas bahan ajar audio visual MYOB dalam pembelajaran Praktika Komputer Akuntansi yang dikembangkan dalam penelitian Tahap I. Target luaran berupa hasil ujicoba lapangan skala kecil dan revisi produk bahan ajar audio visual MYOB, yang dibutuhkan untuk persiapan produk akhir pada penelitian Tahap III. Penelitian menggunakan metode eksperimen dengan disain penelitian One-Shot Case Study dan Intact-Group Comparison. Berdasarkan hasil penelitian dapat disimpulkan bahwa bahan ajar audio visual MYOB efektif digunakan dalam pembelajaran Praktika Komputer Akuntansi dengan tercapainya KKM minimal atau sama dengan 75, walaupun tidak terdapat perbedaan ketuntasan belajar MYOB antara kelompok mahasiswa yang mengikuti pembelajaran Praktik Komputer Akuntansi yang menggunakan Bahan Ajar Audio Visual dan Bahan Ajar Modul cetak.Abstract:This research is aimed to limitedly examine the effectiveness of MYOB audio visual teaching material in Computerized Accounting Practicum which is developed in Phase I. The outcome target is the small-scale try out results and the revision of MYOB audio visual teaching material which are required for  the research in Phase III. This research used experimental methodology with One-Shot Case Study and Intact-Group Comparison research designs. Based on the research result, it can be concluded that MYOB audio visual teaching material is effective in Computerized Accounting Practicum learning with the level of Minimum Mastery Criteria reaches greater than or equal to 75. However, there is no difference in MYOB learning mastery between the group of students who have Computerized Accounting learning with audio visual teaching material and the ones who have printed modul material.
Effect of Piperonyl Butoxide and Silver Nitrate on Degradation Pathways of n-octadecane by Fusarium sp. F092 Hidayat, Asep; Tachibana, Sanro
Indonesian Journal of Forestry Research Vol 3, No 1 (2016): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2016.3.1.%p

Abstract

The aliphatic fraction is usually the largest component in crude oil. Its removal from oil contaminated fields has become an environmental priority and been considered useful for enhancing recovery. Our previous studies reported the isolation of Fusarium sp. F092 based on the ability to degrade chrysene. It also could degrade crude oils and their aliphatics fractions. However, aliphatic degradative pathways in crude oil have not been clearly understood. The identification of aliphatic metabolite pathways using a representative compound n-octadecane was carried out in this study, as well as the effect of Piperonyl Butoxide (PB) and Silver Nitrate (AgNO3) on the degradation of n-octadecane and its metabolite. We determined that Fusarium sp. F092 had ability to break down n-Octadecane from about 125 to 13 mg L-1 after 60 days incubation. During degradation, several metabolite products could be detected and identified to form carboxylic acid groups. By the addition of PB and AgNO3, inhibitor of monooxgenase and dioxygenase enzymes, Fusarium sp. F092 had ability to convert n-octadecane to form alkyl hydroperoxides via terminal oxidation pathway with involving a dioxygenase
DECOLORIZATION OF AZO DYES AND MINERALIZATION OF PHENANTHRENE BY TRAMETES SP. AS03 ISOLATED FROM INDONESIAN MANGROVE FOREST Hidayat, Asep; Tachibana, Sanro
Indonesian Journal of Forestry Research Vol 1, No 1 (2014): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2014.1.1.67-75

Abstract

Textile industry contributes the most disposals of synthetic dyes, and about 40% of textile dyes has been generating high amount of colored wastewater. Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene, is a group of organic compounds, that structurally comprised of two or more benzene rings, which persist in air, water, and soil. The organic pollutants of dyes and PAHs have adversely effects the food chain and are potentially toxic, mutagenic, and carcinogenic to the environment. The objective of this research is to screen and investigate the potential fungus from mangrove forest to degrade azo dyes and phenanthrene.  In this study, fungi were collected from mangrove forest in Riau Province – Sumatra – Indonesia. Previously, Trametes sp. AS03 is one of the fungi isolated from mangrove forest in Riau Province, that was able to decolorize Remazol Brilliant Blue R (RBBR). The capability of Trametes sp. AS03 to decolorize four azo dyes, Remazol B. Violet (V5), Levafix Orange E3GA (Or64), Levafix B. Red E-6BA (R159), and Sumifix S. Scarlet 2GF (R222), were further evaluated. The result shows that Trametes sp. AS03 decolorized 91, 60, 48, and 31 of V5, R222, R159, and Or64, respectively. By showing its capability to decolorize some of the dyes, Trametes sp. AS03 was used to break down phenanthrene. AS03 degraded more than 70% of phenanthrene in 15 days.
PENGARUH UKURAN DIAMETER STEK BATANG Hopea odorata Roxb. DARI KEBUN PANGKAS TERHADAP KEMAMPUAN BERTUNAS, BERAKAR, DAN DAYA HIDUPNYA Hidayat, Asep; Hendalastuti R, Henti; Nurohman, Edi
Jurnal Penelitian Hutan dan Konservasi Alam Vol 4, No 1 (2007): Jurnal Penelitian Hutan dan Konservasi Alam
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Penelitian ini bertujuan untuk mendapatkan informasi tentang besarnya   pengaruh kelas diameter sumber bahan stek Hopea odorata Roxb. terhadap kemampuan membentuk tunas, berakar, dan daya hidup stek. Tanaman sumber bahan stek (ortet) dibagi berdasarkan kelas diameter dan masing-masing kelas diameter dianalisis untuk melihat kelas diameter ortet yang paling produktif menghasilkan stek berkualitas.    Hasil penelitian menunjukkan bahwa kelas diameter ortet berpengaruh sangat nyata terhadap pembentukan jumlah tunas, panjang tunas, dan bahan stek yang dihasilkan. Kemampuan bertunas tertinggi adalah bahan stek asal kelas diameter ortet IV (0,78-0,94 cm) dan V (0,95-1,11 cm) dengan nilai 63 % dan 60 %. Kemampuan berakar dan daya hidup bahan stek dari semua kelas diameter ortet di atas 90 %.
KUALITAS FISIK BIBIT MERANTI TEMBAGA (Shorea leprosula Miq.) ASAL STEK PUCUK PADA TIGA TINGKAT UMUR Junaedi, Ahmad; Hidayat, Asep; Frianto, Dodi
Jurnal Penelitian Hutan dan Konservasi Alam Vol 7, No 3 (2010): Jurnal Penelitian Hutan dan Konservasi Alam
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mendapatkan informasi tentang pertumbuhan dan kualitas fisik bibit meranti tembaga (Shorea leprosula Miq.) asal perbanyakan stek pucuk pada tiga tingkat umur. Penelitian dilakukan melalui pengamatan parameter pertumbuhan dan penilaian mutu fisik bibit meranti tembaga umur sebelas bulan setelah sapih (11 BSS), 12 BSS, dan 14 BSS.  Pengamatan dan penilaian tersebut dilakukan terhadap 10 sampel bibit pada tiap tingkat umur bibit yang dipilih  dengan menggunakan metode penarikan contoh acak sederhana. Hasil penelitian menunjukkan bahwa tinggi bibit umur 14 BSS (40,1 cm) berbeda secara nyata (p < 0,05) dengan umur 11 BSS (32,6 cm) dan 12 BSS (32,6 cm); sedangkan kualitas fisik bibit antartingkat umur tidak berbeda nyata. Pada umur bibit 11 BSS bibit sudah siap tanam dengan tinggi 32,6 cm; kekokohan 10,79; rasio pucuk akar 2,58; dan indeks mutu bibit 0,28
EFFECT OF PIPERONYL BUTOXIDE AND SILVER NITRATE ON DEGRADATION PATHWAYS OF N-OCTADECANE BY Fusarium sp. F092 Hidayat, Asep; Tachibana, Sanro
Indonesian Journal of Forestry Research Vol 3, No 1 (2016): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2016.3.1.1-7

Abstract

The aliphatic fraction is usually the largest component in crude oil. Its removal from oil contaminated fields has become an environmental priority and been considered useful for enhancing recovery. Our previous studies reported the isolation of Fusarium sp. F092 based on the ability to degrade chrysene. It also could degrade crude oils and their aliphatics fractions. However, aliphatic degradative pathways in crude oil have not been clearly understood. The identification of aliphatic metabolite pathways using a representative compound n-octadecane was carried out in this study, as well as the effect of Piperonyl Butoxide (PB) and Silver Nitrate (AgNO3) on the degradation of n-octadecane and its metabolite. We determined that Fusarium sp. F092 had ability to break down n-Octadecane from about 125 to 13 mg L-1 after 60 days incubation. During degradation, several metabolite products could be detected and identified to form carboxylic acid groups. By the addition of PB and AgNO3, inhibitor of monooxgenase and dioxygenase enzymes, Fusarium sp. F092 had ability to convert n-octadecane to form alkyl hydroperoxides via terminal oxidation pathway with involving a dioxygenase