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PCS-4 IN VIVO EMBRYO PRODUCTION AT CIPELANG LIVESTOCK EMBRYO CENTRE Satrio, Faisal Amri; Karja, Ni Wayan Kurniani; Imron, M; Siswani, Yuni; Parlindungan, Oloan; Purwantara, Bambang
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.888 KB)

Abstract

Livestock Embryo Center/Balai Embrio Ternak (BET) Cipelang is one of the government institution under the supervision of Directorate General of Livestock and Animal Health Services, Ministry of Agriculture of the Republic of Indonesia. BET Cipelang has the main task of carrying out the production, development, and distribution of livestock embryo for all regions of Indonesia. BET Cipelang produced embryos using two methods, there are in vivo and in vitro. The produced embryos will be transferred to the recipient cow using embryo transfer technique.                Embryo transfer biotechnology can be used to enhance genetic improvement and to increase marketing opportunities with purebred cattle. Because of their relatively low reproductive rate and long generation interval, embryo transfer is especially useful with cattle. The success of embryo transfer depends on factors associated with the embryo, the recipient or an interaction among the factors of the embryo and recipient [1].                 Embryo quality is one of the main factors for successful embryo production. In vivo embryo production has better quality than in vitro such as cell number, morphology, growth and development ability and after freezing.  The frequency of occurrence of chromosomal abnormalities in in vivo-derived embryos are lower than in vitro and the consequence is that only 30-40% of oocytes resulting from in vitro maturation develop into blastocysts after in vitro fertilization and the in vitro yield rate of embryos are lower than from in vivo [2]. Recently, there are no studies about the performance of in vivo embryo production at BET Cipelang. The aim of this study is to explore the quality of in vivo embryos produced by BET Cipelang.
VERIFICATION TO THE RSG-GAS FUEL DISCHARGE BURN-UP USING SRAC2006 MODULE OF COREBN/HIST Susilo, Jati; Sembiring, Tagor Malem; Imron, M; Sunaryo, Geni Rina
JURNAL TEKNOLOGI REAKTOR NUKLIR TRI DASA MEGA Vol 20, No 1 (2018): Februari 2018
Publisher : Pusat Teknologi Dan Keselamatan Reaktor Nuklir (PTKRN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4760.282 KB) | DOI: 10.17146/tdm.2018.20.1.4041

Abstract

For 30 years operation, some of the modifications to the RSG GAS core has been done, that are changes included the type of fuel from U3O8-Al to U3Si2-Al with the same density 2.96 gU/cc, the loading pattern of standard fuel elements/fuel control elements from 6/1 & 6/2 to 5/1 pattern, and in core fuel management calculation tool has been change from IAFUEL to BATAN-FUEL. To obtain an extension of the operating license for the next 10 years, the RSG-GAS Periodic Safety Assessment Document is need to prepared. According to the Regulatory Body Chairman Regulation No. 2 2015, RSG-GAS safety assessment should be done independently. As part of this assessment the fuel discharge burn-up must be estimated. In this research, to ensure that the misposition of fuel element in the core has not occurred, the investigation to the document operating report related the fuel placement has been done. Therefore, by using 78th to 93rd operation data, verify of the fuel discharge burn-up of the RSG-GAS has been performed by using SRAC2006 module of COREBN/HIST. In addition, the results of these calculations are also made comparative with the operating report data that is calculated by using BATAN-FUEL. Maximum fuel discharge burn-up (57.73% of U-235) was verified still under permissible value determined by the regulatory body (<60% of U-235). Maximum differences value between two computer codes was about 2.12 % of U-235 (3.80%) that is fuel at the B-7 position. Fuel discharge burn-up of RSG-GAS showed almost the same value for each the operation cycle, range of 1.52% of U-235. So it can be concluded that the RSG-GAS core operation over the last ten years was in good fuel management performance, in accordance with the design. BATAN-FUEL has been comformed well enough with COREBN/HIST. Keywords: Discharge Burn-Up, RSG-GAS, COREBN/HIST, BATAN-FUEL Verifikasi Terhadap Burn-Up Buang Bahan Bakar Teras RSG-GAS Menggunakan SRAC2006 Modul COREBN/HIST. Selama 30 tahun beroperasi, RSG-GAS telah mengalami perubahan modifikasi antara lain jenis bahan bakar dari U3O8-Al menjadi U3Si2-Al dengan kerapatan sama 2,96 gU/cc, pola pemuatan bahan bakar standar/elemen kendali dari pola 6/1 & 6/2 menjadi pola 5/1, dan alat perhitungan manajemen bahan bakar IAFUEL dengan BATAN-FUEL. Untuk memperoleh perpanjangan ijin operasi selama 10 tahun ke depan, maka perlu disiapkan dokumen Penilaian Keselamatan Berkala RSG-GAS. Berdasarkan PerKa BAPETEN No. 2 Tahun 2015, maka penilaian keselamatan RSG-GAS harus dilakukan secara independen. Salah satu parameter yang perlu diverifikasi adalah nilai bahan bakar buang. Dalam penelitian ini, dilakukan investigasi terhadap dokumen Laporan Operasi untuk memastikan bahwa tidak terjadi kesalahan penempatan bahan bakar. Selanjutnya, berdasarkan data siklus operasi teras ke 78 sampai dengan 93, dilakukan verifikasi nilai burn-up buang bahan bakar RSG-GAS dengan menggunakan SRAC2006 modul COREBN/HIST. Selain itu, hasil perhitungan tersebut juga dilakukan komparasi dengan data laporan operasi yaitu data hasil perhitungan menggunakan BATAN–FUEL. Fraksi bakar buang bahan bakar terbesar (57,73% U-235) terverivikasi masih di bawah nilai limit yang ditetapkan oleh badan pengawas (<60% U-235). Perbedaan hasil perhitungan terbesar kedua program computer sebesar 2,12% U-235 (3,80%) yaitu pada posisi B-7. Fluktuasi burn-up buang bahan bakar menunjukkan nilai yang hampir sama untuk tiap-tiap siklus operasi, jarak (range) sebesar 1,52% U-235. Sehingga dapat disimpulkan bahwa operasi teras RSG-GAS selama sepuluh tahun terakhir menunjukkan performa manajemen bahan bakar yang baik, sesuai desain. BATAN-FUEL telah terkonfirmasi cukup baik dengan COREBN/HIST. Kata kunci: Burn-up buang, RSG-GAS, COREBN/HIST, BATAN-FUEL
Viability of bovine demi embryo after splitting of fresh and frozen thawed embryo derived from in vitro embryo production Imron, M; Boediono, A; Supriatna, I
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.55 KB) | DOI: 10.14334/jitv.v12i2.549

Abstract

In vivo embryo production was limited by number of donor, wide variability respond due to superovulation program and also immunoactifity of superovulation hormone (FSH). Splitting technology could be an alternative to increase the number of transferrable embryos into recipien cows. Splitting is done with cutting embryo becoming two equal pieces (called demi embrio) base on ICM orientation.  The objective of this research was to determine the viability of demi embryo obtained from embryo splitting of fresh and frozen thawed embryo. The results showed that demi embryos which performed blastocoel reexpansion 3 hours after embryo splitting using fresh and frozen thawed embryos were 76.9 and 76.2% respectively. Base on existention of inner cell mass (ICM), the number of demi embryos developed with ICM from fresh and frozen thawed embryos were not significantly different (90.6 and 85.7% respectively. The cell number of demi embryo from fresh embryos splitting was not different compared with those from frozen thawed embryos (36.1 and 35.9 respectively). These finding indicated that embryo splitting can be applied to frozen thawed embryos with certain condition as well as fresh embryos. Key Words: In Vitro Embryo, Splitting, Demi Embryo, Cell Number
Growth hormone gene family (GH, GHR, GHRH and Pit-1) polymorphisms and its association with superovulation response, ovulation rate and embryo quality in Embryo Transfer Station (BET) of Cipelang Sumantri, Cece; Imron, M; ., Sugyono; Andreas, E.; Restu, M.; Ishak, A.B.L.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 2 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v16i2.642

Abstract

The decrease in fertility is considered to be the main cause of reproductive loss in dairy cattle and beef industry. Many candidate genes that play an important role in fertility and embryonic development. The purpose of this study was to detect genetic variations of the growth hormone gene family (GH|MspI, GH|AluI, GHR| AluI, GHRH|HaeIII and Pit-1|HinfI) and its association with superovulation response, ovulation, fertilization and transferable embryos rate. A total of 45 blood samples taken from cows that have been superovulated Angus, Brahman, HF, Limousin and Simmental. DNA was extracted with phenol-chloroform protocol followed by polymerase chain reaction technique (PCR) using specific primers for GH, GHR, GHRH and Pit-1 gene. PCR product was cut with restriction enzyme MspI, AluI, HaeIII and HinfI and electrophoresed on agarose gel and stained with ethidium bromide (EtBr). Superovulation is done by injecting a totally of 20 ml FSH for 4 days. Injecting the prostaglandin hormone (PGF2α) was performed on the eleventh day of CIDR implantation. Artificial insemination (AI) performed two or three days after the injection of PGF2α and Flushing was done on the seventh day after the AI.  The results showed that the Angus, Limousin, Brahman and Simental GH loci diversity of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI was not associated with superovulation response, ovulation, fertilization and transferable embryo rate. In HF dairy cattle, genotype on Pit-1|HinfI AA has higher percentage of superovulation response (P &lt; 0.05) when compared to AB genotype, but did not differ to BB genotype. Dairy cattle HF AA genotype also had higher ovulation rate (P &lt; 0.05) when compared to AB and BB genotypes, but AB and BB have the same ovulation rate. Key Words: Polymorphisms, Growth Hormone Genes and Reproduction traits.
Viability of bovine demi embryo after splitting of fresh and frozen thawed embryo derived from in vitro embryo production Imron, M; Boediono, A; Supriatna, I
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.55 KB) | DOI: 10.14334/jitv.v12i2.549

Abstract

In vivo embryo production was limited by number of donor, wide variability respond due to superovulation program and also immunoactifity of superovulation hormone (FSH). Splitting technology could be an alternative to increase the number of transferrable embryos into recipien cows. Splitting is done with cutting embryo becoming two equal pieces (called demi embrio) base on ICM orientation.  The objective of this research was to determine the viability of demi embryo obtained from embryo splitting of fresh and frozen thawed embryo. The results showed that demi embryos which performed blastocoel reexpansion 3 hours after embryo splitting using fresh and frozen thawed embryos were 76.9 and 76.2% respectively. Base on existention of inner cell mass (ICM), the number of demi embryos developed with ICM from fresh and frozen thawed embryos were not significantly different (90.6 and 85.7% respectively. The cell number of demi embryo from fresh embryos splitting was not different compared with those from frozen thawed embryos (36.1 and 35.9 respectively). These finding indicated that embryo splitting can be applied to frozen thawed embryos with certain condition as well as fresh embryos. Key Words: In Vitro Embryo, Splitting, Demi Embryo, Cell Number
Growth hormone gene family (GH, GHR, GHRH and Pit-1) polymorphisms and its association with superovulation response, ovulation rate and embryo quality in Embryo Transfer Station (BET) of Cipelang Sumantri, Cece; Imron, M; ., Sugyono; Andreas, E.; Restu, M.; Ishak, A.B.L.
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 2 (2011): JUNE 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v16i2.642

Abstract

The decrease in fertility is considered to be the main cause of reproductive loss in dairy cattle and beef industry. Many candidate genes that play an important role in fertility and embryonic development. The purpose of this study was to detect genetic variations of the growth hormone gene family (GH|MspI, GH|AluI, GHR| AluI, GHRH|HaeIII and Pit-1|HinfI) and its association with superovulation response, ovulation, fertilization and transferable embryos rate. A total of 45 blood samples taken from cows that have been superovulated Angus, Brahman, HF, Limousin and Simmental. DNA was extracted with phenol-chloroform protocol followed by polymerase chain reaction technique (PCR) using specific primers for GH, GHR, GHRH and Pit-1 gene. PCR product was cut with restriction enzyme MspI, AluI, HaeIII and HinfI and electrophoresed on agarose gel and stained with ethidium bromide (EtBr). Superovulation is done by injecting a totally of 20 ml FSH for 4 days. Injecting the prostaglandin hormone (PGF2α) was performed on the eleventh day of CIDR implantation. Artificial insemination (AI) performed two or three days after the injection of PGF2α and Flushing was done on the seventh day after the AI.  The results showed that the Angus, Limousin, Brahman and Simental GH loci diversity of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI was not associated with superovulation response, ovulation, fertilization and transferable embryo rate. In HF dairy cattle, genotype on Pit-1|HinfI AA has higher percentage of superovulation response (P &lt; 0.05) when compared to AB genotype, but did not differ to BB genotype. Dairy cattle HF AA genotype also had higher ovulation rate (P &lt; 0.05) when compared to AB and BB genotypes, but AB and BB have the same ovulation rate. Key Words: Polymorphisms, Growth Hormone Genes and Reproduction traits.
Kinerja kelembagaan program sanitasi lingkungan berbasis masyarakat (SLBM) Kurniasih, Denok; Setyoko, Paulus Israwan; Imron, M
Masyarakat, Kebudayaan dan Politik Vol 29, No 1 (2016): Masyarakat, Kebudayaan dan Politik
Publisher : FISIP Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.562 KB) | DOI: 10.20473/mkp.V29I12016.14-21

Abstract

SLBM Program implementation in Banyumas Regency involves many stakeholders, one of them is community. Through KSM, community has authority to manage program from, beside government roles as facilitator. Thus, institutional system is required to support performance of KSM. By using descriptive qualitative approach, this research aimed to analyze institutional performances of KSM and aspects which is able to support and inhibit the institutional performance of KSM. This research found that institutional performance of KSM has not been able to reach policy goals. This can be seen from community participation in SLBM program. Program’s effectiveness is also not optimal. Thus, there have been no changes in the community habits of sanitation-related issues. Moreover, some of support and inhibit aspects of institutional performance of KSM has indentified.
Strategi Kolaborasi Pengembangan Wisata Berbasis Edukasi di Clungup Mangrove Conservation Desa Tambakrejo Kabupaten Malang Imron, M; Anwar, M Saiful
Journal of Governance Innovation Vol 1 No 1 (2019): Volume 1 Nomor 1 Tahun 2019 (Maret 2019)
Publisher : Program Studi Ilmu Pemerintahan Universitas Islam Raden Rahmat Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36636/jogiv.v1i1.299

Abstract

Abstrak Pengembangan wisata alam berbasis edukasi memiliki tantangan yang serius di ranah mempertahankan eksistensi dan keberlanjutan lingkungan. Setiap stakeholder yang ada harus berani menjamin keterlibatan mereka dapat memberi kontribusi yang positif bagi keberlangsungan ekosistem kawasan pantai. Model Quintuple Helix memberikan posisi yang signifikan bagi lingkungan alami masyarakat untuk memperoleh perhatian yang lebih. Berangkat dari realitas, penelitian ini bertujuan untuk mencari strategi yang ideal bagi kolaborasi pengembangan wisata berbasis edukasi. Penelitian ini dilakukan dengan metode deskriptif kualitatif, pengambilan data dilakukan dengan observasi, wawancara dan studi dokumen. Adapun hasil dari penelitian ini berupa konsep strategi kolaborasi yang ideal untuk pengembangan wisata berbasis edukasi dengan model Quintuple Helix. Melalui model ini dapat memberikan modal tambahan berupa manajemen berbasis kualitas pembangunan yang efektif, keseimbangan alam yang terjamin, serta garansi terhadap generasi mendatang agar tetap dapat merasakan keberlanjutan lingkungan. Abstract Educational tourism-based natural tourism development has serious challenges in the realm of maintaining the existence and sustainability of the environment. Every existing stakeholder must have the courage to guarantee that their involvement can contribute positively to the sustainability of the coastal ecosystem. The Quintuple Helix model provides a significant position for the natural environment of the community to get more attention. Departing from reality, this study aims to find the ideal strategy for collaborative education-based tourism development. This research was conducted with a qualitative descriptive method, data collection was carried out by observation, interviews and document studies. The results of this study are in the form of a collaboration strategy concept that is ideal for education-based tourism development with the quintuple helix model. Through this model, it can provide additional capital in the form of effective quality-based development management, guaranteed natural balance, and guarantees against. &nbsp;
KAPASITAS BISNIS PEMERINTAH DESA DALAM PERSPEKTIF BUSINESS-GOVERNMENT RELATIONSHIP PASCA IMPLEMENTASI OTONOMI DESA (KASUS DI KABUPATEN BANYUMAS) Kurniasih, Denok; Israwan Setyoko, Paulus; Imron, M
Transparansi : Jurnal Ilmiah Ilmu Administrasi Vol 3, No 1: Juni 2020
Publisher : Institut Ilmu Sosial dan Manajemen STIAMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (631.856 KB) | DOI: 10.31334/transparansi.v3i1.927

Abstract

The Indonesian Governments policies after the implementation of village autonomy are directed to villages business development. Village governments duties and functions are not only related to administrative matters anymore, but also in operation of villages business. This shows that there is a developing business commitment in Indonesia. The research result shows that the Business-Government Relationship in Indonesia has changed from one way to mutual interaction, as shown with the development of Public Sector Entrepreneurship and business commitment in village government environment. Both factors evidently improve the capacity of business, which begins to be developed by the Indonesian Government at all levels. The development of Public Sector Entrepreneurship is shown with the business creativity, business cooperation, and professionalism of village government in governments business management. Meanwhile, local governments business commitment is shown with support of policies, funding and human resources in governments business management. Governments business capacity is shown with efficiency, effectiveness and economy (value for money) achieved by governments business unit. In Indonesia, particularly in Banyumas Regency, village autonomy has given opportunity for the development of Business-Government Relationship at local level.