Endah Retno Palupi
Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor, Jln. Meranti Kampus IPB, Dramaga, Bogor 16680

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Pengaruh Tingkat Kematangan Buah terhadap Setek Basal Daun Mahkota Nanas (Ananas comosus (L.) Merr) Cv. Smooth Cayenne Eprilian, Husna Fatima; Suhartanto, Mohamad Rahmad; Palupi, Endah Retno
Jurnal Hortikultura Indonesia Vol. 10 No. 2 (2019): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.989 KB) | DOI: 10.29244/jhi.10.2.75-84

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Propagation method of pineapple that can be developed to increase the number of seedling is crown leaf bud cuttings method. The level of fruit maturity is thought to affect the successfull of crown leaf bud cuttings. This study was aimed to obtain information about the effect of fruit maturity level when taking the crown for cutting material for pineapple cv. Smooth Cayenne seedling production. The experiment used a RCBD with one factor, the factor is the level of maturity with four levels of treatment: Level of maturity 1 (K1): all eyes were green; maturity 2 (K2): yellow fruit eyes <20%; maturity 3 (K3): yellow fruit eyes 40-55%; and maturity 4 (K4): yellow fruit eyes> 90%; but the reddish-orange color <20%. Each treatment was repeated five times, so there were 20 experimental units. The results showed that the levels of endogenous auxin and cytokines were not different at all levels of maturity stage, but the C / N ratio of K3 and K4 showed higher results. All maturity stage showed maximum bud formation at 4-6 WAP. The best fruit maturity level for pineapple seedling production with basal leaf crown cuttings is K4 (yellow fruit eyes> 90%, but reddish orange <20%). K4 had trend to produce higher survived, sprouted cutting and seedling dry weight. K4 showed the highest shoot dry weight and adequate seedling certification standards faster. Keyword: dormant bud, fast propagation, Smooth Cayyene, split crown, tropical fruit
Pengaruh Suhu Ruang Simpan dan Perlakuan Pasca Penyimpanan terhadap Mutu dan Produktivitas Umbi Benih Bawang Merah (Allium cepa L. group Aggregatum) Sarjani, Alvita Sekar; Palupi, Endah Retno; Suhartanto, Muhammad Rahmad; Purwanto, Y. Aris
Jurnal Hortikultura Indonesia Vol. 9 No. 2 (2018): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340 KB) | DOI: 10.29244/jhi.9.2.111-121

Abstract

ABSTRACTThe fluctuative price of shallot in Indonesia is mainly due to discontinuous supply. Shallot is usually planted three times a year. Lack of supply occurs during July to October. Therefore, the produce need to be stored to ensure its availability during off season, not only for consumption but also as seed bulb for the following planting season. The research was aimed to maintain the quality of seed bulbs during twelve weeks storage and to evaluate productivity of the seed bulbs after storage. Shallot seed bulbs of Bima Brebes was used for the research that was devided into two steps. The first step was arranged in nested design, in which seed bulb was stored at 0 0C, 5 0C, 10 0C and ambient temperature nested into storage period i.e 0, 3, 6, 9 and 12 weeks with four replications. The second step was evaluation of productivity of the seed bulbs that was arranged in nested design. The seed bulbs, after being stored at diferent condition, was subjected to different acclimatization treatments i.e. gradual increase of temperatures for 3 days and direct change to ambient temperature for one day, to devernalize the seed bulbs and replicated our times. The results showed that the dormant period of shallot seed bulbs lasted for 8 weeks after harvest (6 week after storage) as indicated by germination and vigor index of >90%. The termination of dormancy coincided with a rise in GA, IAA and cytokines as well as ABA. Storing the seed bulbs for 3 months in 5 0C could maintain its viability and vigor >90%, with 9.8% of total damage and 15.6% of weight loss. The seed bulbs grew normally and produced 30.2 g of bulb per plant. The percentage of flowering plant of gradually acclimatized seed bulbs previously stored at 5 0C (10.3%) was not significantly different from those directly acclimatized at ambient temperature (12%).Keywords: ABA, cytokinin, dormancy, GA, weight lossABSTRAKPenyebab utama fluktuasi harga bawang merah di pasar adalah ketersediaan umbi bawang merah yang tidak stabil. Di daerah sentra produksi, bawang merah ditanam tiga kali dalam setahun. Bulan Juli sampai Oktober adalah periode hasil panen rendah. Penyimpanan umbi merupakan salah satu upaya untuk menjamin ketersediaannya di luar musim panen, tidak hanya untuk konsumsi tetapi juga memastikan ketersediaan umbi sebagai benih pada musim tanam selanjutnya. Penelitian ini bertujuan mempertahankan kualitas benih umbi selama 12 minggu disimpan dan mengevaluasi produktivitasnya setelah penyimpanan. Bahan yang digunakan adalah benih umbi bawang merah varietas Bima Brebes. Penelitian dibagi dalam dua tahap. Tahap pertama adalah penyimpanan benih umbi dirancang dalam nested design yang mana benih umbi disimpan pada suhu 0 0C, 5 0C, 10 0C dan suhu ruang tersarang pada waktu penyimpanan yang terdiri atas 0, 3, 6, 9 dan 12 minggu dan diulang empat kali. Tahap kedua adalah evaluasi produktivitas benih umbi setelah disimpan dirancang dalam nested design. Umbi yang telah disimpan (12 minggu) pada masing-masing kondisi simpan diberi perlakuan aklimatisasi, yaitu suhu berjenjang (3 hari) dan suhu ruang langsung (1 hari), untuk mencegah pembungaan. Setiap perlakuan diulang sebanyak empat kali (ulangan tersarang pada aklimatisasi). Hasil penelitian menunjukkan bahwa benih umbi mengalami dormansi sampai 8 minggu setelah panen (6 minggu setelah simpan), ditandai dengan daya berkecambah dan indeks vigor di atas 90%. Berakhirnya dormansi benih umbi bersamaan dengan peningkatan kandungan giberelin, auksin, dan sitokinin mengimbangi peningkatan asam absisat. Penyimpanan benih selama 12 minggu pada suhu 5 0C dapat mempertahankan viabilitas dan vigor di atas 90% dengan kerusakan (umbi bertunas, chilling injury, hampa atau busuk) sebesar 9.8% dan susut bobot sebesar 15.6%. Setelah disimpan selama 12 minggu benih dapat tumbuh normal dan memproduksi 30.2 g umbi per tanaman. Aklimatisasi suhu berjenjang umbi benih yang telah disimpan pada suhu 5 0C menghasilkan persentase pembungaan (10.3%) yang tidak berbeda nyata dengan aklimatisasi suhu ruang langsung (12%).Kata kunci: ABA, dormansi, GA, sitokinin, susut bobot
Peningkatan Pembungaan dan Hasil Biji Bawang Merah Varietas Bima melalui Vernalisasi dan Aplikasi GA3 Fahrianty, Dian; Poerwanto, Roedhy; Widodo, Winarso Drajad; Palupi, Endah Retno
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.42 KB) | DOI: 10.18343/jipi.25.2.245

Abstract

The low production of true seed of shallot (TSS) is mostly caused by a low flowering rate. The research was aimed to increase the flowering rate and seed yield in lowland (240 masl) and highlands (1250 masl) by vernalization and submersion of the seed bulbs in GA3 before planting. The research was arranged in a completely randomized block design with 2 factors and replicated four times. The first factor was vernalization, i.e. without and with vernalization at 10±2°C for 30 days, and the second factor was the submersion of the seed bulbs following vernalization in GA3 at the concentrations of 0, 100, and 200 ppm for 1 h. Parameters measured were time of 50% flowering (days after planting), percentage of flowering plants, number of umbel per plant, number of floret per umbel, fruitset, seed weight per umbel and per plant, as well as the percentage of seed germination. The result suggested that when production of TSS of var. Bima is to be carried out in the lowland then the seed bulbs had to be vernalized at 10±2°C for 30 days which will increase the flowering percentage and seed yield. If the production of TSS is to be carried out in the highland then vernalization at 10±2°C for 30 days will increase the flowering percentage and seed yield. However, if for any reason vernalization is impossible to proceed then the submersion of the seed bulbs in 100 ppm GA3 for 1 h was sufficient to increase the flowering percentage and seed yield. The produced seeds (TSS) either in the low as well as in the highlands had high germination percentages. Keywords: germination, highland, lowland, umbel, true seed of shallot
Penyimpanan Serbuk Sari Jagung dan Potensinya untuk Produksi Benih Hibrida Fariroh, Indri; Palupi, Endah Retno; Suwarno, dan Faiza Chairani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 2 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.285 KB) | DOI: 10.24831/jai.v45i2.12535

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Production of maize hybrid seed is hindered by relatively poor production by the male line pollen; short live of the pollen which was ascribed to desiccation and dehydration sensitive and flowering asynchrony between male and female lines. The aims of this study were to enhance production and viability of male line pollen and also to determine the suitable storage condition for maintaining pollen viability in order to be used for production of hybrid seed. The research consisted of two experiments. The first experiment was arranged in split plot randomized block design with NPK dosages as main plot (0, 300, 600, 900 kg ha-1) and application of boron as sub-plot (0, 1.5, 3, 4.5, 6 kg ha-1), replicated three times. The second experiment used randomized block design with two factors, i.e. storage temperature (-196 oC and -20 oC) and storage period (0, 1, 2, 3, 4 weeks). The result showed that NPK 900 kg ha-1 increased the number of spike per tassel from 9.3 to 10.7. Application of NPK 600 kg ha-1 or boron 1.5 kg ha-1 improved pollen viability. Combination of NPK 900 kg ha-1 and boron 6 kg ha-1 resulted in the highest pollen viability of 95.3%. Pollen after being stored for four weeks either in -196 ºC or -20 ºC was possible to be used for seed production with 20% seed set.Keywords: boron, freezer, liquid nitrogen, NPK dosage, pollen viability  
Efektivitas Bahan Pelapis Benih terhadap Penyerapan Fosfat dan Pertumbuhan Bibit Kelapa Sawit ,, Saipulloh; Palupi, Endah Retno; Widajati, Eny; Mathius, dan Nurita Toruan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.151 KB) | DOI: 10.24831/jai.v45i1.12236

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One way of overcome phosphate deficiency in oil palm plantation is seed enrichment using phosphate solvent microbes. This technique is expected to accelerate phosphate availability for plant. The aim of the research was to identify the best coating materials for seed enrichment that compatible with Burkholderia sp. in order to increase phosphate absorption, seed storability, and growth of oil palm seedlings. This research consisted of two consecutive experiments. The first experiment was arranged in randomized block design, with nine treatments of different seed coating materials. The second experiment was arranged in nested design, with main factor of 11 different seed coating materials and nested factors were five storage periods. The data was collected on growth parameters of seedling height, root length, trunk height, dry weight; phosphate absorption, and DGGE analysis. The research showed there were three best seed coating materials of CMC 1.5%, CMC 2% + gypsum 1.5%, and CMC 1.5% + talc 1%. The coating material enhances seedling growth and phosphate absorption. Seed coating and enrichment maintained seedling vigor for three days in the storage. Keywords: Burkholderia sp., carboxyl methyl cellulose, phosphate solvent microbial, seed enrichment, seed storage
Flower Initiation, Morphology, and Developmental Stages of Flowering-Fruiting of Mindi Syamsuwida, Dida; Palupi, Endah Retno; Siregar, Iskandar Zulkarnaen; Indrawan, Andry
Jurnal Manajemen Hutan Tropika Vol. 18 No. 1 (2012)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (696.252 KB)

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The study was aimed to determine flower initiation, floral morphology and to observe the stages of flowering and fruit  development of mindi (Melia azedarch  L) within a population  for one period of time 2008–2009.  The methods used were observing directly over the trees and some vegetatives and generatives buds were sampled for dissecting.  The observation revealed that the inflorescence type of mindi was panicle, located at the end of a branch.  The number of flower varied among inflorescences, ranged between 30–80 that bloomed simultaneously.  The flower was hermaphroditic with position of anther was closed to stigma that selfing might be happened.  Usually, the ovary contained 5 ovules that  developed into seeds.  Reproductive cycle was proceeded for 6–7 months within the year, first observation commenced from flower initiation that occur in August, generative buds to flower burst  in September–October.  Early fruits were formed in October–November and fruits reached physiological-maturity in January–February.  Reproductive success was 34%, indicated that the rate of fertilized ovules proportion to be potencially viable seeds were relatively low.
Peningkatan vigor kelapa sawit melalui pengayaan kecambah dengan Trichoderma asperellum, Cendawan Mikoriza Arbuskular dan Enterobacter sacchari Increasing of oil palm seedling vigor through seed enrichment with Trichoderma asperellum, Arbuscular Mycorrhizal Fungi, and Enterobacter sacchari) UTAMI, Esty Puri; WIDAJATI, Eny; PALUPI, Endah Retno; TORUAN-MATHIUS, Nurita
E-Journal Menara Perkebunan Vol 86, No 2 (2018): Oktober 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1019.312 KB) | DOI: 10.22302/iribb.jur.mp.v86i2.267

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Oil palm is a leading commodity of the plantation sector in Indonesia. Improving the quality of oil palm still be carried out to increase production. Seed technology can be used as an effort to improve the quality of oil palm seeds. The aim of this experiment was to determine the effect of seed enrichment with consortium of three microbes to increase vigor of oil palm seedling in pre nursery stage. The experiment design of this reseach was using completely randomize block design consisted of two factors. The first was seed coating consist of two factors, ie: coated seed and uncoated seed. Second was seed enrichment consist of eight factors, ie: control, enrichment with E. sacchari, abruscular mycorrhizal fungi (AMF), T. asperellum, E. sacchari+ AMF, E. sacchari+ T. asperellum, AMF + T. asperellum, E. sacchari+ AMF + T. asperellum. The result showed that enrichment with consortium of three microbes could increase vigor of oil palm seedling based on seedling germination, rate of germination, palm height, and numbers of survival seedling.[Keywords:biological agent, compatibility, diazotroph] Abstrak *) Penulis korespondensi: eny.widajati61@gmail.com Kelapa sawit adalah komoditas unggulan sektor perkebunan di Indonesia. Peningkatan mutu kelapa sawit terus dilakukan agar mening-katkan produksinya. Teknologi benih dapat digu-nakan sebagai salah satu upaya peningkatan mutu benih kelapa sawit. Penelitian ini bertujuan untuk menetapkan pengaruh pengayaan konsorsium tiga mikroba, E. sacchari,T. asperellumdan cendawan mikoriza arbuskular (CMA) dan pelapisan kecambah terhadap peningkatan vigor bibit kelapa sawit di pre nursery. Percobaan dirancang dengan rancangan acak kelompok dengan dua faktor, yaitu pelapisan dan pengayaan. Pelapisan terdiri dari dua taraf, yaitu dengan pelapisan dan tanpa pelapisan. Pengayaan terdiri dari 8 taraf, yaitu kontrol, pengayaan dengan E. sacchari, CMA, T. asperellum, E. sacchari+ CMA, E. sacchari + T. asperellum,CMA + T.asperellum,E. sacchari+ CMA + T. asperellum. Hasil penelitian menunjukkan bahwa pengayaan dengan konsor-sium tiga mikroba dapat meningkatkan vigor bibit kelapa sawit berdasarkan parameter daya tumbuh, kecepatan tumbuh, tinggi bibit, dan jumlah bibit yang hidup.  [Kata kunci:    agen hayati,    diazotrop, kompa-tibilitas]
Kajian Karakter Ketahanan terhadap Cekaman Kekeringan pada Beberapa Genotipe Bibit Kelapa Sawit (Elaeis guineensis Jacq.) Palupi, Endah Retno; Dedywiryanto, Yopy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.979 KB) | DOI: 10.24831/jai.v36i1.1341

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The research was aimed to study the response of oil palm seedlings to drought stress and to determine characters which would be useful in selection for drought resistance. This research was conducted at PT Dami Mas Sejahtera field station and SMART Research Institute laboratory Libo, Riau from April to August 2005. The research was arranged in split plot design with two factors in randomized block design. The main plot was soil water content, i.e. 100%FC/field capacity : 24+1%; 75%FC : 18+1%; 50%FC : 12+1%; and 25%FC : 6+1%, whereas the subplot was four genotypes: G1: 635xAP.01, G2: 635x742.316, G3:15x742.316 and G4: 15xAP.01. The result showed that only soil water content as low as 25%FC could significantly inhibit seedling growth, as indicated by reduction of leave water content,  shoot and root dry weight, seedling height, root length and volume; and an increase of water deficit. Soil water content ranging from 50-100% FC did not give any significant effect. Based on the physiological responds, G1 and G3 were relatively more resistant to drought stress than G2 and G4. Leaf water content was the easiest, cheapest, fastest and non-destructive variable to be used for early selection of drought resistant oil palm seedlings.   Key words:  Oil palm, seedling, character for selection, drought resistant
Evaluation of Growth, Flowering and Seed Morphology of Batflower, Tacca chantrieri Andre Ajisyahputra, Nikko Rizky; Palupi, Endah Retno; Krisantini, Krisantini; Wiendi, Ni Made Armini; Mulyono, Joko
Journal of Tropical Crop Science Vol 4 No 2 (2017): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (970.221 KB)

Abstract

Tacca chantrieri is a perennial plant belongs to Taccaceae family that has the potential to be marketed as an ornamental plant due to its unique shape and dark color inflorescence. To date, very limited publication on T. chantrieri are available. The aim of this research was to examine plant growth pattern and flower phenology of T. chantrieri from West Borneo, Indonesia, and from Queensland, Australia in relation to their potential development as ornamental pot plants. The study was extended to examine T. chantrieri seed morphology with an inclusion of an accession from Thailand.  The field experiment was conducted at the Leuwikopo experimental station in Darmaga, Bogor, Indonesia. Seed morphology examination was conducted at the Histology Laboratory of Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. T. chantrieri from West Borneo blooms six times a year whereas the Queensland accession twice a year. West Borneo accession had smaller bractea, fewer numbers of flowers per inflorescence and shorter flower stalk than Queensland accession. In addition, T. chantrieri Queensland grew faster than Borneo with the one leaf formed every two to three weeks, whereas it was at least four weeks in the Borneo accession. Both accessions were susceptible to leaf brown spot disease caused by Cercospora taccae. T. chantrieri Queensland seeds are oval dark brown, whereas West Borneo and Thailand accessions were shaped like kidneys (reniform) with a lighter brown color. This study demonstrated that there is great diversity in T. chantrieri including plant morphology, seed shape and color, which are important for species identification and for development of T. chantrieri as ornamental pot plant.
Oil Palm Seed Coating with Enriched Trichoderma asperellum (T13) to Suppress Infection of Ganoderma boninense Pat. Jawak, Gani; Widajati, Eny; Palupi, Endah Retno; Mathius, Nutrita Toruan
Jurnal Perbenihan Tanaman Hutan Vol 6, No 2 (2018): Jurnal Perbenihan Tanaman Hutan
Publisher : Forest Tree Seed Technology Research & Development Center (FTSTRDC)/ Balai Penelitian dan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.582 KB) | DOI: 10.20886/bptpth.2018.6.2.121-132

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The attack Ganoderma boninense can caused stem rot of oil palms that occur at all stage of plant growth. Trichoderma asperellum endophytic can suppressed the attack of Ganoderma in a nursery by utilizing seed coating technology. The aim of this study was to determine the best formula of seed coating materials which is compatible with T. asperellum (T13). The first experiment consisted of two phases, namely, the first phase was testing the effectiveness and compatibility of T. asperellum through a mixing technique of  T. asperellum suspension with coating material. The best three result on the first phase (25 percent arabic gum, 1 percent CMC, and 3percent arabic gum + 1 percent gypsum) were used in the second phase of the experiment, which was testing the effectiveness and compatibility of T. asperellum through soaking technique in T. asperellum suspension that continued by coating. The three best result types of formulas from the second phase (1 percent CMC, 1.5 percent CMC, and 4,5 percent arabic gum + 1,5 percent gypsum) were used for the second experiment, namely testing the resistance of oil palm seed on Ganoderma. The results show that 25 percent arabic gum, 1 percent carboxy methyl cellulose (CMC), 1 percent arabic gum + 1 percent gypsum have a potency as coating of materials. The best formula for seed coating is soaking with T. asperellum + coating 1 percent CMC, 1.5 percent  CMC and arabic gum 4.5 percent + gypsum 1.5 percent. In addition, soaking seeds with T. asperellum + coating 1.5 percent CMC can enhance the ability of seed to grow up to 16.67 percent compared to the control, but did not effective in suppressing G. boninense infection during pre-nursery stage.