Agus Eko Prasetyo
Pusat Penelitian Kelapa Sawit, Medan

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Laju fotosintesis pada tanaman kelapa sawit terinfeksi karat daun Cephaleuros virescen Susanto, Agus; Prasetyo, Agus Eko; Priwiratama, Hari; Syarovi, Muhdan
Jurnal Fitopatologi Indonesia Vol 16 No 1 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.235 KB) | DOI: 10.14692/jfi.16.1.21-29

Abstract

Photosynthesis Rate of Oil Palm Infected by Red Rust Cephaleuros virescens The incidence of oil palm red rust disease caused by Cephaleuros virescens tends to increase in Indonesia. However, the loses due to this disease has never been measured quantitatively. This study was conducted to measure the rate of photosynthesis on 5-year-old palms with 3 infection categories, i.e. mild, medium, and heavy infection. Field observation of oil palm in Kalianta showed that the infection of red rust disease occurred starting from lower to the upper fronds. Higher infection was prevalently on the adaxial than abaxial leaf. The red rust infection on frond number 17 or younger severely affects the photoshynthesis rate of the oil palm. On the palm with heavy infection category, the photosynthesis rate on frond number 9 was decreased up to 42.48%, i.e. from 15.51 µmol to 8.92 µmol, compared to that with mild infection.
Laju Infeksi Ganoderma pada Empat Kelas Tekstur Tanah Susanto, Agus; Prasetyo, Agus Eko; Wening, Sri
Jurnal Fitopatologi Indonesia Vol 9 No 2 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.255 KB) | DOI: 10.14692/jfi.9.2.39

Abstract

Basal stem rot disease caused by Ganoderma is the most destructive disease in oil palm plantation in Indonesia. Development of this disease is faster and heavier in nutrient poor soils, such as sandy soil. The objective of this research is to study and quantitatively measure the infection rate of Ganoderma disease in sandy soil. The treatments consisted of five different mixtures of mineral soil (M) and sand (P) i.e: A (100% M–0% P), B (75% M-25% P), C (50% M-50% P), D (25% M-75% P), and E (0% M-100% P). Physical, chemical, and biological properties of each soil mixture treatment were analyzed. Soil texture can be differentiated into sandy clay loam, sandy loam, loamy sand, sand for treatmen A, B, C, D, E, respectively. Sandy soil medium had less microorganism population (< 1 × 106 cfu g-1soil) than sandy clay loam which microrganism population was 3 × 106 cfu g-1 soil. The infection rate of Ganoderma in sandy soils was 1.77–1.83 palm per month per 100 palms. Infection rate of Ganoderma in sandy soil was faster and higher than those in loamy soilKey words: basal stem rot, oil plam, sandy soil
Ganoderma boninense Penyebab Penyakit Busuk Batang Atas Kelapa Sawit Susanto, Agus; Prasetyo, Agus Eko; Priwiratama, Hari; Wening, Sri; Surianto, Surianto
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.51 KB) | DOI: 10.14692/jfi.9.4.123

Abstract

Basal stem rot disease (BSR) caused by Ganoderma boninense is the major pathogen of oil palm in Indonesia and Malaysia. Sometimes in the same location, especially in the peatland, upper stem rot (USR) disease is also found. This research was carried out to identify the pathogen of upper stem rot by molecular approach. Fruiting bodies of fungus were collected from 3 plants with upper stem rot symptoms and 2 plants with basal stem rot symptoms from Labuhan Batu North Sumatra and Kampar Riau. Results showed that all of the fruiting body samples taken from plants with upper stem rot and basal stem rot symptoms were G. boninense.
Respons Curvularia lunata Penyebab Penyakit Bercak Daun Kelapa Sawit terhadap Berbagai Fungisida Susanto, Agus; Prasetyo, Agus Eko
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.166 KB) | DOI: 10.14692/jfi.9.6.165

Abstract

Leaf spot disease of oil palm caused by Curvularia sp. is the major disease in nursery. Implementation of best nursery practices is the key to prevent it. Generally, fungicides are used only if epidemic of leaf spot diseases occur in the field. The objectives of this research were to determine causal agent of leaf spot disease of oil palm and the potential alternative weed host around the nursery, to select suitable fungicides, and to study the effect of fungicides rotation to disease incidence. The results showed that the causal agent of leaf spot disease of oil palm was Curvularia lunata. The fungus was also found on grasses, Cyperus rotundus and Imperata cylindrica. Difeconazol, copper oxide, and propineb suppressed leaf spot disease in nursery. Application of fungicide by rotation between difeconazol and copper oxide with frequency every 10 days suppressed the development of leaf spot disease of oil palm in the nursery.
Pengendalian Penyakit Busuk Pangkal Batang Kelapa Sawit secara Kultur Teknis Priwiratama, Hari; Prasetyo, Agus Eko; Susanto, Agus
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.722 KB) | DOI: 10.14692/jfi.10.1.1

Abstract

Effort to manage basal stem rot disease (BSR) caused by Ganoderma boninense could be conducted through several cultural practice approaches. Four series of field experiments was conducted to observe the effectiveness of cultural practice methods for controlling the epidemic of basal stem rot due to G. boninense. Sanitation trial consisted of three treatments i.e root sanitation with big hole planting system, big hole without root sanitation, and standard replanting system as comparison. Hole in hole and standard planting system were compared in the hole in hole trial. Digging and mounding trial was conducted in four treatments, i.e. digging and mounding with teer and Trichoderma, digging and mounding with Trichoderma, mounding with Trichoderma, and infected palm without any treatment as comparison. Meanwhile, a 4 m × 4 m trench was used in the isolation trench trial. Annual observation was conducted to determine the disease incidence of BSR in each trial. Results showed that all methods could prevent palms from G. boninense infection in the early stage of oil palm development. Proper root sanitation delayed G. boninense infection until 2 years after treatment (YAT), hole in hole planting system was able to suppress disease incidence up to 7 YAT, digging and mounding was useful to prolong the life of the infected palms up to 3 YAT, meanwhile the use of isolation trench was demonstrated to prevent G. boninense infection to neighboring palms up to 2 YAT.
Deteksi dan Identifikasi Fitoplasma yang Berasosiasi dengan Penyakit Layu Kelapa Di Pulau Derawan, Kalimantan Timur Prasetyo, Agus Eko; Mutaqin, Kikin Hamzah; Giyanto, Giyanto
Jurnal Fitopatologi Indonesia Vol 13 No 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (979.473 KB) | DOI: 10.14692/jfi.13.3.89

Abstract

Coconut is a major commodity in Derawan island as source of additional income for the farmers. Research was conducted to detect and identify phytoplasmas associated with coconut wilt disease in Derawan island. Coconut wilt disease was indicated by the typical symptoms, i.e.  leaf yellowing, shorten of coconut sheaths and leaves, necrosis and collapse of old leaves, and also nut fall.  The presence of phytoplasmas in phloem tissues of coconut stem was observed using fluorescence and electron microscope.  Identification of phytoplasmas was carried out by nested-PCR and sequencing of the 16S rRNA gene.  DNA fragment of phytoplasma with the size of 1.25 kbp was successfully amplified using primer pairs P1/P7, followed by primer pairs R16F2n/R16R2.  Sequence analysis of the amplified fragments showed that phytoplasma associated with coconut wilt disease in Derawan island belongs to 16SrII (witches broom phytoplasma) and 16SrXI (ca. Phytoplasma oryzae) groups.
KARAKTERISASI FITOPLASMA PENYEBAB PENYAKIT LAYU KELAPA DI PULAU DERAWAN MENGGUNAKAN RFLP IN SILICO Prasetyo, Agus Eko; Mutaqin, Kikin Hamzah; ., Giyanto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.705 KB) | DOI: 10.23960/j.hptt.217105-110

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Characterization of phytoplasmas associated with coconut wilt disease in Derawan Island using in silico RFLP. Coconutwilt disease has been reported in Derawan Island that resulted in eradication up to 10% of the total cultivated palms. Theobjective of this study was to detect and characterize phytoplasmas associated with coconut wilt disease in Derawan islandusing nested PCR technique and in silico RFLP based on 16S rRNA gene sequences. Detection of phytoplasmas was performedusing nested PCR method, cloning of nPCR products, sequencing, and analysis of sequencing results using in silico RFLP.The results revealed that phytoplasmas could not be detected by PCR using P1/P7 primer pairs however it could be amplifiedby nested PCR using R16F2n/R16R2 primer pairs resulting amplicon at about 1.25 kb. In silico RFLP analysis indicated thatphytoplasmas associated with coconut wilt disease in Derawan Island belong to 16SrII (witches broom phytoplasma). PCRproduct of the nPCR need to be sequenced because the R16F2n/R16R2 primer will also amplify the other bacterial species, mainly from Gram positive bacteria.
PERKEMBANGAN POPULASI Elaeidobius kamerunicus FAUST PASCA INTRODUKSI DAN PENINGKATAN FRUIT SET KELAPA SAWIT DI PULAU SERAM, MALUKU, INDONESIA Prasetyo, Agus Eko; Susanto, Agus
Jurnal Penelitian Kelapa Sawit Vol 24 No 1 (2016): Indonesian Journal of Oil Palm Research
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.747 KB) | DOI: 10.22302/iopri.jur.jpks.v24i1.7

Abstract

Elaeidobius kamerunicus Faust merupakan serangga penyerbuk utama pada perkebunan kelapa sawit di Indonesia saat ini. Pengembangan perkebunan kelapa sawit di areal yang baru memerlukan introduksi serangga ini terutama jika areal baru tersebut berada di pulau yang berbeda dan dibatasi oleh lautan yang cukup luas. Upaya pengiriman E. kamerunicus telah dilakukan dari Sumatra Utara ke Pulau Seram, Maluku. Introduksi E. kamerunicus dilakukan pada stadia larva dan kepompong di dalam tandan bunga jantan 4-5 hari lewat mekar. Pengiriman ke Pulau Seram merupakan introduksi pertama kali yang ditunjukkan dengan tidak dijumpainya serangga ini pada bunga jantan maupun betina kelapa sawit dan nilai fruit set kelapa sawit yang sangat rendah sebesar 11,27%. Introduksi awal sekitar 500 kumbang/ha dapat berkembang menjadi 362.401 kumbang/ha dalam waktu 5 bulan dengan sex ratio kelapa sawit sekitar 97%. Satu bulan pasca introduksi, fruit set kelapa sawit yang dihasilkan dengan populasi tersebut mencapai 75,56%. Semakin rendah sex ratio kelapa sawit atau semakin banyak ketersediaan bunga jantan kelapa sawit maka semakin cepat perkembangan populasi kumbang E. kamerunicus dan semakin banyak jumlah kumbang yang berkunjung ke bunga betina mekar sehingga semakin tinggi nilai fruit set kelapa sawit yang dihasilkan.
OPTIMASI PROTOKOL EKSTRAKSI DNA Elaeidobius kamerunicus Wening, Sri; Prasetyo, Agus Eko; Rozziansha, Tjut Ahmad Perdana; Susanto, Agus
Jurnal Penelitian Kelapa Sawit Vol 24 No 2 (2016): Indonesian Journal of Oil Palm Research
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.677 KB) | DOI: 10.22302/iopri.jur.jpks.v24i2.10

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Serangga penyerbuk kelapa sawit (Elaeidobius kamerunicus Faust) memiliki peran penting pada tingkat produktivitas perkebunan kelapa sawit di Indonesia. Hingga saat ini, belum ada penelitian yang cukup komprehensif mengenai biologi spesies tersebut pada tingkat molekuler. Padahal, pengetahuan dasar tersebut sangat berguna bagi pengembangan serangga penyerbuk kelapa sawituntuk tujuan efektivitas pembentukan fruit set kelapa sawit. Penelitian ini bertujuan untuk mendapatkan protokol ekstraksi DNA E. kamerunicus yang sangat berguna bagi analisis sidik jari DNA spesies tersebut. Hasil menunjukkan bahwa pada penggunaan suatu kit ekstraksi DNA, material sampel yang dihancurkan terlebih dahulu dengan micro pestle tanpa penambahan larutan penyangga menghasilkan DNA dengan kuantitas tertinggi, sementara perlakuan lain yang menggunakan tissue lyser sebagai penghancur material tidak memiliki perbedaan kuantitas DNA yang nyata. DNA yang diperoleh baik dengan cara menggunakan micro pestle atau tissue lyser untukpenghancuran material menghasilkan DNA yang memadai untuk analisis sidik jari DNA menggunakan teknik AFLP (Amplified Fragment Length Polymorphism) dan sekuensing.
KEEFEKTIFAN FUNGISIDA TERHADAP ISOLAT CENDAWAN TERBAWA BENIH KELAPA SAWIT Simanjuntak, Donnarina; Faizah, Rokhana; Prasetyo, Agus Eko; Susanto, Agus
Jurnal Penelitian Kelapa Sawit Vol 25 No 1 (2017): Indonesian Journal of Oil Palm Research
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.465 KB) | DOI: 10.22302/iopri.jur.jpks.v25i1.24

Abstract

Benih kelapa sawit diketahui dapat membawa beberapa mikroba yang bersifat patogenik dan menurunkan mutu benih. Penelitian bertujuan untuk mengetahui keefektifan bahan aktif fungisida kimia dan nantinya sebagai bahan rekomendasi pengendalian cendawan terbawa benih. Penelitian dilakukan di laboratorium Proteksi Tanaman, PPKS Unit Usaha Marihat, Sumatra Utara, pada April hingga Oktober 2016. Penelitian menggunakan analisis deskriptif benih dan metode pengujian kesehatan benih yang digunakan terdiri atas pengamatan visual benih kering dan teknik pencucian benih (washing test). Benih diperoleh dari 6 lokasi produksi yaitu pengupasan benih, seleksi benih, perendaman 1, penganginan 1, ruang perkecambahan, dan penyaluran benih. Fungisida yang digunakan terdiri atas 39 jenis bahan aktif yang berbeda. Dari hasil pengujian kesehatan benih terdapat tiga cendawan terbawa benih yang berhasil diisolasi yaitu Aspergillus, Penicillium, dan Mucor. Ketiga cendawan ini selanjutnya diuji terhadap 39 jenis fungisida dan hasilnya terdapat 13 sampai 18 fungisida yang efektif menekan laju pertumbuhan ketiga cendawan tersebut hingga hari ke-7 setelah aplikasi.