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Polimorfisme Penanda RAPD untuk Analisis Keragaman Genetik Pinusmerkusii di Hutan PendidikanUnhas Gusmiaty, Gusmiaty; Restu, Muh.; Asrianny, Asrianny; Larekeng, Siti Halimah
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.822 KB) | DOI: 10.31258/jnat.16.2.47-53

Abstract

The aims of Genetic study of pinus identified stand in Unhas Experimental Forest is to analyses of genetic characteristics of stand, based on RAPD (Random Amplified Polymorphic DNA) marker. The study was conducted in Biotechnology and Tree Breeding Laboratory, Forestry Faculty, Hasanuddin University. The method are DNA isolation, Primers selection and RAPD analyses. DNA analyses of Pine with ten RAPD marker showed number of alel varietied and polymorphic. Coefficient of similarity in population have number of 0.15-0.73.Highest genetic distance is 0.9630 and lowest genetic distance is 0.2698. Number of genetic diversity of Pine in Experimental Forest Hasanuddin University is 0,489 and categorized highly.
OPTIMASI KOMBINASI NAA, BAP DAN GA3 PADA PLANLET KENTANG SECARA IN VITRO Larekeng, Siti Halimah
JURNAL GALUNG TROPIKA Vol 1, No 1 (2012)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.93 KB) | DOI: 10.31850/jgt.v1i1.32

Abstract

The research held in Agricultural Biotechnology Laboratorium of Hasanuddin University Research Center on January-October 2009.  This research aimed to know the effect of NAA, BAP and GA3 combination to potato growth in vitro.  We hope that the result could be use as information to develop qualified potato seed production through in vitro. This research used Complete Randomized Design with NAA, BAP and GA3 combination treatment.  The treatments were  kontrol, NAA 0,01 mg L-1 + BAP 0,05 mg l-1 +  GA3  0,1 mg l-1 , NAA 0,01 mg l-1 + BAP 0,05 mg l-1 +  GA3  0,2 mg l-1 ), R3 (NAA 0,01 mg l-1 + BAP 0,1 mg l-1 +  GA3  0,1 mg l-1 ), R4 (NAA 0,01 mg l-1 + BAP 0,1 mg l-1 +  GA3  0,2 mg l-1 ), R5 (NAA 0,1 mg l-1 + BAP 0,05 mg l-1 +  GA3  0,1 mg l-1 ), R6 (NAA 0,1 mg l-1 + BAP 0,05 mg l-1 +  GA3  0,2 mg l-1 ), R7 (NAA 0,1 mg l-1 + BAP 0,1 mg l-1 +  GA3  0,1 mg l-1 ), R8 (NAA 0,1 mg l-1 + BAP 0,1 mg l-1 +  GA3  0,2 mg l-1 ).  Each treatment consists of 2 experiment units.  Each treatment has 3 replication so there were 54 experiment units. The result showed that NAA 0,1 mg l-1 + BAP 0,1 mg l-1  + GA3 0,1  mg l-1 combination gave the best potato growth in number of twigs, number of leaves and planlet height.
POLYMORPHISM OF SIMPLE SEQUENCE REPEAT REGIONS OF SULAWESI EBONY (DIOSPHYROS CELEBICA BAKH.) IN EXPERIMENTAL FOREST OF HASANUDDIN UNIVERSITY PROVENANCE Larekeng, Siti Halimah; Restu, Muh.; Gusmiaty, Gusmiaty; Rismawati, Rismawati
AgroTech Journal Vol 1, No 1 (2016): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2168.934 KB) | DOI: 10.31327/atj.v1i1.173

Abstract

Polymerase Chain Reaction (PCR)-based molecular techniques have been used to detect the polymorphism in plants. The utilization of molecular markers plays essential role in germplasm characterization and plant breeding since the information of DNA marker technology can be exchanged between laboratories and should have standard method to be reproducible. The molecular aspect has been commonly linked to DNA isolation protocol and polymorphic molecular marker, thus can be used for molecular research recommendation purposes. The objectives of this study were to evaluate the capability of microsatellite marker of Ebenaceae Family for amplifying Ebony DNA, and to determine the appropriate PCR annealing temperatures. The DNA isolation of Ebony leaves from Experimental Forest of Hasanuddin University Provenance was carried out using Genomic DNA Mini Kit (Plant) Geneaid protocol. Nine of seventeen selected primers from the Genus Diospyros were able to amplify Ebony DNA. Amplification products produced polymorphic bands with different annealing temperatures (ranged from 53 to 56°C). These nine polymorphic primers will be recommended to use for future studies in genetic diversity as well as pollen dispersal pattern analyses.
Identifikasi dan Karakterisasi Mikoriza pada Tegakan Nyatoh (Palaquium sp.) Kurnia, Kurnia; Gusmiaty, Gusmiaty; Larekeng, Siti Halimah
PERENNIAL Vol 15, No 1 (2019)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v15i1.6850

Abstract

This research aimed to identify the mycorrhiza types that associated in roots and soil on  Palaquium sp. plant. This  research has implemented on December 2017 until February 2018. This research procedures made by two stages includes collecting soil and root samples on Palaquium sp plant on  Ko'mara Community Forest, Takalar District. Therefore, the second step is isolation, identification, and observing colonization of mycorrhiza spores in the Integrated Laboratory and Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Hasanuddin University, Makassar. The results showed types of spore that found were Glomus, Gigaspora and Acaulospora. Glomus spore has highest relative abundance in root samples whereas on soil samples were Glomus, Gigaspora, and Schleroderma with Glomus spores has highest relative abundance in. The colonization percentage on site was intermediate.
KARAKTERISTIK STRUKTUR ANATOMI DINDING SEL JABON MERAH (Anthocephalus macrophyllus) PROVENANSI WAJO Mompewa, Nurul Muhlisah; Yunianti, Andi Detti; Larekeng, Siti Halimah
PERENNIAL Vol 15, No 1 (2019)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v15i1.6496

Abstract

Apart from being a key to identifying wood, the characteristics of the anatomical structure of cell walls can be used as a reference in determining the quality of wood, especially for its use. However, the characteristics of wood in the same species are sometimes found to be different due to the growing place is unidentical. Therefore, this study aimed to identify the cell wall characteristics of Jabon Merah wood from Wajo provenance. The wood was collected from Tangkoro Village, Pitumpanua District, Wajo Regency, South Sulawesi. It was then prepared in slicing and maceration samples. The structures of cell walls observed in the preparations were a pit, perforation plate, and tylosis. The results showed the Jabon Merah had inter vessel pit and perforation plate types, namely polygonal alternating and scalariform, respectively. Also, it had a tylosis in the vessel.
POLLEN DISPERSAL PATTERNS EBONI LASITAE PROVENANCE BASED ON SIMPLE SEQUENCE REPEATS (SSR) Restu, Muhammad; ., Gusmiaty; Larekeng, Siti Halimah
Jurnal Silvikultur Tropika Vol 7 No 3 (2016): Jurnal Silvikultur Tropika (Suplemen Desember)
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Parentage analysis has been used to evaluate pollen dispersal in ebony (Diosphyros celebica Bakh.). The objectives of this research were to evaluate (i) the dispersal of pollen, and (ii) the distance of pollen travel in ebony Lasitae provenances. The finding of this activities should be beneficial to support breeding of this ebony tree. There were 14 progeny arrays were harvested from 3 female parents. There were 32 ebony trees surrounding the female parents were analyses as the potential male parents for the progenies. Ebony tress were mapped according to their GPS position. All samples were genotyped using four SSR marker loci. Parentage analysis was done using CERVUS version 2.0 software. Results of the analysis indicated the evaluated markers were effective for assigning candidate male parents to all evaluated seedlings. There is no specific direction of donated pollen movement from assigned donor parents to the female ones. The donated pollens could come from assigned male parents in any directions relative to the female parent positions. Pollen dispersal pattern of ebony female parent occured in outcrossing pollination among its different donated trees. Based on the progenies analysis, ebony female parent is a dominantly outcrossing pollination species. Distances of pollen travel reach to 18 until 269 meter.Key words: Ebony, ssr marker, pollen dispersal.
Pemanfaatan Penginderaan Jauh Dalam Penilaian Keberhasilan Reklamasi di Lahan Pasca Tambang PT. Vale Indonesia Nursaputra, Munajat; Larekeng, Siti Halimah; Nasri, Nasri; Hamzah, Andi Siady; Mustari, Andi Subhan; Arif, Abdur Rahman; Ambodo, Aris Prio; Lawang, Yohan; Ardiansyah, Andri
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 11 No 1 (2021): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.11.1.39-48

Abstract

Mining activities with an open system trigger land degradation which results in a decrease in land quality. The decline in land quality is related to the level of fertility and soil chemical properties, so that in general ex-mining land contains low nutrients. These problems in several mining companies that implement environmental sustainability are resolved through reclamation activities. This reclamation activity needs to be assessed, to measure the success of the mine in overcoming land degradation problems. In this study we demonstrate an assessment of the success of mine reclamation in the largest nickel mining area in South Sulawesi, using remote sensing technology. Formulation of NIR and Red bands on Sentinel 2 imagery can produce Normalized Difference Vegetation Index index. From the vegetation index value, it is known that the observed reclamation area is close to the high vegetation index value (0.7 - 0.9). This value is actually close to the vegetation index value in the surrounding natural forest, but with a low percentage of area. The distribution of the results of the assessment of plant growth in the reclamation area was lowest vegetation by 3.14%; lower vegetation by 12.15%; low vegetation by 21.53%; moderate vegetation by 14.82%; high vegetation by 25.94% and higher vegetation by 22.42% of the total reclamation area.
KERAGAMAN GENETIK IKAN KAKAP PUTIH (Lates calcarifer Bloch, 1790) TIPE LIAR DAN DOMESTIKASI MENGGUNAKAN METODE Random Amplified Polymorphic DNA (RAPD) ., Irmawati; Parawansa, Basse Siang; A.R Tassakka, Asmi Citra Malina; Baharuddin, Ma’rifa; Larekeng, Siti Halimah
JFMR (Journal of Fisheries and Marine Research) Vol 5, No 1 (2021): JFMR VOL 5 NO.1
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.01.15

Abstract

Kegiatan pembesaran ikan kakap putih di beberapa Kabupaten di Sulawesi Selatan yang menggunakan benih yang diproduksi dari balai perikanan di Sulawesi Selatan dan Ambon telah berkembang selama beberapa tahun terakhir. Akan tetapi, benih yang dihasilkan dari kegiatan pembenihan tersebut memiliki kualitas yang masih rendah yang diduga karena keragaman genetik yang rendah. Penelitian ini menggunakan metode Random Amplified Polymorphic DNA, RAPD untuk menganalisis variasi genetik ikan kakap putih tipe liar dari Bulungan Kalimantan Utara dan ikan hasil domestikasi dari panti pembenihan di Ambon yang dibesarkan di tambak rakyat di Desa Borongkalukua Kabupaten Maros Sulawesi Selatan. Hasil penelitian menunjukkan bahwa ikan kakap putih tipe liar memiliki nilai heterozigositas yang lebih tinggi dibandingkan dengan ikan kakap putih hasil domestikasi. Jarak genetik antar individu-individu di dalam populasi tipe liar lebih tinggi dibandingkan dengan jarak genetik individu-individu hasil domestikasi. Dendogram dan pohon filogenetik yang dibentuk berdasarkan evolutionary dissimilarity menunjukkan bahwa pada tingkat ketidakmiripan 10% ikan kakap putih tipe liar dari Bulungan Kalimantan Timur membentuk klaster yang terpisah dengan ikan kakap putih hasil domestikasi dari Ambon yang dipelihara di tambak Dusun Borongkalukua Kabupaten Maros. Keragaman genetik ikan hasil domestikasi lebih rendah dibandingkan ikan tipe liar dan menunjukkan pola evolusi yang pholyphyly Hasil yang diperoleh tersebut menunjukkan bahwa marka RAPD dapat digunakan untuk menganalisis perubahan keragaman genetik antara populasi ikan kakap putih tipe liar dan hasil domestikasi serta bermanfaat sebagai informasi dalam menyusun strategi pemanfaatan wild population sebagai sumber induk dan konservasi plasma nutfah ikan kakap putih.