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The oxidation reaction of quinine by potassium permanganate (KMnO4) Rosalina, Reny; Alni, Anita; Mujahidin, Didin; Santoso, Joko
Jurnal Penelitian Teh dan Kina Vol 18, No 2 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (588.892 KB) | DOI: 10.22302/pptk.jur.jptk.v18i2.76

Abstract

Quinine was known widely as malarial drug and a bitter taste ini soft drink. Recently, quinine have been also applied in organo-catalysis process, separation and purification of chiral compounds from enantiomeric mixture by diasteromeric salt crystallization and chiral chromatography. Quinine contains various functional groups which easily transforms by oxidations. This research was to study the oxi-dation reaction of quinine by using potassium permanganate (KMnO4) as oxidator. The KMnO4 oxidation carried out under acidic con-ditions. The separation and purification of pro-ducts were done by radial chromatography with chloroform : metanol : tri ethyl amine (9:1:0.1) as eluent. The structure of products were cha-racterized by spectroscopic data including: in-frared spectroscopy, NMR, and mass spectro-metry as quinine-1-N-oxide and quininal.
Molecular Mechanism of Cholerae Toxin (ctx) in Causing Diarrhea Praja, Rian Kusuma Arinta; Rosalina, Reny
Oceana Biomedicina Journal Vol 1, No 2 (2018): Oceana Biomedicina Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (761.654 KB) | DOI: 10.30649/obj.v1i2.16

Abstract

Vibrio cholerae is one of the pathogenic bacteria transmitted through contaminated food, especially seafood and contaminated beverages. V. cholerae produces cholerae toxin (ctx) which is encoded by the ctx gene located within its chromosome. This toxin has been recognized as one of the toxins responsible for cholera outbreaks. The mechanism of ctx gene expression is induced by environmental signals such as pH, osmolarity, temperature, bile, amino acids, and CO2. These signals will be a positive transcriptional factor to the ToxR gene that regulates the biogenesis of cholerae toxin. After cholerae toxin has been successfully expressed, V. cholerae uses a type II secretion (T2S) pathway to deliver cholerae toxin to the extracellular environment. Cholerae toxin consists of A and B subunits. The B subunit plays a role in attaching to the receptor Manosialosyl Ganglioside (GM1 ganglioside) and the A subunit plays a role in catalyzing ADP-ribosylation of Gs (stimulatory) proteins and turning them into active condition. The Gs proteins will convert the inactive adenilate cyclase (AC) into active AC. The increase of AC activity will increase the cyclic adenosine 3'5'-monophosphate (cAMP) concentration along the cell membrane. The cAMP then causes the active secretion of sodium (Na+), chloride (Cl-), potassium (K+), bicarbonate (HCO3-), and water (H2O) out of the cell into the intestinal lumen, resulting in large fluid losses and electrolyte imbalances. Keywords: Vibrio cholerae, cholerae toxin (ctx), ToxR gene, type II secretion (T2S), GM1 ganglioside, adenilate cyclase.
Aktifitas Antibakteri Ekstrak Jamur Endofit Mangga Podang (Mangifera indica L.) Asal Kabupaten Kediri Jawa Timur Rosalina, Reny; Ningrum, Riska Surya; Lukis, Prima Agusti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.006 KB) | DOI: 10.20884/1.mib.2018.35.3.757

Abstract

Jamur endofit merupakan salah satu mikroba endofit yang hidup di dalam jaringan tumbuhan dan diketahui dapat menghasilkan senyawa aktif yang mirip dengan tumbuhan inangnya. Penelitian ini bertujuan untuk mengetahui aktifitas antibakteri dari ekstrak media dan ekstrak jamur endofit yang berhasil diisolasi dari ranting mangga podang (Mangifera indica L.). Didapatkan empat jenis jamur endofit hasil isolasi yang kemudian dikultur menggunakan media padat Potato Dextrose Agar (PDA) kemudian dipindahkan ke media cair Potato Dextrose Broth (PDB). Setelah miselia tumbuh selama inkubasi, media dan biomassaa jamur dipisahkan dengan filtrasi vakum. Jamur yang telah dikeringkan dimaserasi menggunakan metanol, sedangkan media jamur diekstraksi menggunakan etil asetat. Ekstrak jamur dan ekstrak media kemudian diuji aktivitas terhadap bakteri Escherichia coli, Staphylococcus aureus, Salmonella thypi, dan Staphylococcus mutan menggunakan metode difusi cakram kertas. (Disk Diffusion Method). Kontrol negatif yang digunakan adalah aquades sedangkan sebagai pembanding adalah antibiotik ciprofloxacin dan kloramfenikol. Hasil penelitian menunjukan bahwa ekstrak jamur dan ekstrak media jamur memiliki aktifitas sebagai antibakteri dengan sensitifitas yang berbeda-beda. Ekstrak media jamur memiliki sensitifitas yang lebih tinggi terhadap beberapa macam bakteri dibandingkan dengan ekstrak yang diperoleh dari biomassa jamur.
Pelatihan Pengolahan Limbah Ampas Nanas Sebagai Produk Pengempuk Daging Rosalina, Reny; Ramadani, Aisyah Hadi; Ningrum, Riska Surya
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 3 NOMOR 2 SEPTEMBER 2019 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.116 KB) | DOI: 10.30595/jppm.v3i2.5176

Abstract

Nanas merupakan potensi pertanian yang dihasilkan sangat melimpah tiap tahunnya di Dusun Puhrejo, Kecamatan Ngancar, Kabupaten Kediri. Buah nanas hasil panen selain dijual dalam bentuk buah segar juga diolah menjadi sari nanas. Dari produksi sari nanas dihasilkan limbah ampas nanas yang belum dimanfaatkan. Tujuan dari kegiatan ini adalah untuk meningkatkan pengetahuan tentang pengolahan limbah ampas nanas sebagai pengempuk daging sehingga mendorong kemandirian ekonomi masyarakat melalui pengolahan produk berbasis potensi daerah. Target capaian dalam program ini adalah meningkatkan pengetahuan masyarakat tentang pemanfaatan limbah ampas buah nanas, bertambahnya varian produk olahan buah nanas, serta meningkatnya keinginan masyarakat untuk berwirausaha produk olahan nanas. Metode yang digunakan yaitu melalui penyuluhan dan pelatihan kepada warga Dusun Puhrejo. Hasil kegiatan yaitu  pengetahuan masyarakat tentang potensi nanas dan pengolahan limbah ampas nana meningkat,  serta keinginan dan kesadaran masyarakat untuk  mengolah nanas  menjadi produk yang bervariasi meningkat.  Hal tersebut juga mendorong masyarakat untuk membentuk kelompok usaha untuk mengembangkan produk olahan berbahan nanas sebagai produk UMKM dusun Puhrejo.
THE EXISTENCE OF VIBRIO CHOLERAE IN INDONESIA: FROM ENVIRONMENTAL TO CLINICAL ASPECTS (A CONCISE REVIEW) Ka Praja, Rian; Pusporini, Anggita Ratri; Rosalina, Reny; Arta, I Wayan Muda Suta; Sukrama, I Dewa Made; Fatmawati, Ni Nengah Dwi
Jurnal PPI Dunia Vol 4 No 1 (2021)
Publisher : OISAA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vibrio cholerae is an infectious agent causing cholera disease with a high prevalence in various developing countries. V. cholerae is a pathogen with broad spectrum host that can infect humans and animals, especially aquaculture. The existence of this disease in Indonesia has long been identified in several outbreaks. Various reports in Indonesia have succeeded in finding the existence of V. cholerae in the environment, aquaculture, food and beverage, as well as in clinical cases of V. cholerae infection. The presence of V. cholerae in environment such as water source is commonly related with contamination. However, V. cholerae can be found in aquatic environment as this environment is natural habitat for V. cholerae. Thus, aquaculture is prone to be infected with V. cholerae because the presence of this pathogen is abundant in aquatic environment. Contaminated food and beverage are associated with hygiene and sanitation and human is commonly infected after consuming contaminated food or beverage. This brief review has the main focus to discuss the existence of V. cholerae from environmental to clinical aspects found in Indonesia.
Perancangan primer gen lktB pada Fusobacterium necrophorum untuk analisis PCR Praja, Rian Ka; Rosalina, Reny
Jurnal Sains dan Teknologi Peternakan Vol 2 No 2 (2021): Vol 2 No 2 (2021): Jurnal Sains dan Teknologi Peternakan
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/jstp.v2i2.960

Abstract

Fusobacterium necrophorum is a pathogen causing disease in animals, especially cattle, goats, and sheep. F. necrophorum infection can result in a variety of necrotic conditions (necrobacillosis). This study aimed to design a pair of primers for detecting the leukotoxin B (lktB) gene expressed by F. necrophorum as diagnostic support. The lktB gene sequence was obtained from GenBank NCBI with accession number AF312861.3:685-2337. Furthermore, the sequence was used as a template for in silico primer design using Primer-BLAST. Primer candidates successfully designed were then analyzed for their secondary structure using NetPrimer. The results showed that forward primer set 6 (5'-TCGGATGCTGGAATGCTACTT-3') and reverse primer set 6 (5'-GGGCTCCCAAATCCTTACGA-3') were a favorable primer set with a product size of 228 bp. However, laboratory experiments need to be carried out to determine the optimal conditions for this primer set.
Pelatihan pembuatan minuman berserat alami dari buah nanas dan nata de coco Ramadani, Aisyah Hadi; Rosalina, Reny; Ningrum, Riska Surya
JPPM (Jurnal Pendidikan dan Pemberdayaan Masyarakat) Vol 7, No 2 (2020): November 2020
Publisher : Departement of Nonformal Education, Graduate Scholl of Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jppm.v7i2.32162

Abstract

Salah satu sentra produksi nanas di Jawa Timur adalah di Dusun Puhrejo, Kecamatan Ngancar, Kabupaten Kediri. Nanas yang dihasilkan dijual dalam bentuk buah segar dan olahan, contohnya adalah sari nanas. Kualitas produk olahan sari nanas masih kurang maksimal sehingga pada kegiatan ini diberikan pengetahuan dan pelatihan tentang cara pengolahan buah nanas dalam bentuk minuman berserat. Minuman berserat dibuat dari campuran buah nanas yang kaya nutrisi dan nata de coco yang mengandung serat alami. Penambahan nata de coco juga berfungsi sebagai penstabil (emulsifier) agar produk minuman yang dihasilkan memiliki mutu yang lebih baik dan daya simpan yang lebih lama. Terdapat 2 tahapan kegiatan yang diikuti oleh 25 warga Dusun Puhrejo, yaitu penyuluhan dan pelatihan. Hasil yang diperoleh dari kegiatan ini adalah adanya peningkatan pengetahuan peserta mengenai pemanfaatan dan cara pengolahan nanas yang tepat sehingga tidak mengurangi kandungan gizi di dalamnya. Minat peserta untuk mengembangkan produk olahan nanas juga meningkat karena produk yang dihasilkan dapat memberi nilai tambah buah nanas. Produk olahan yang diperoleh dari kegiatan ini juga dijadikan produk unggulan UMKM Dusun Puhrejo. Training on produce a healthy beverage from pineapple fruits and nata de coco AbstractOne of the pineapple production centers in East Java is in Puhrejo village, Ngancar, Kediri. The pineapple was sold in the fresh and processing fruits, like pineapple juice. But the pineapple juice has low quality so this activity gave information and training about how to process pineapple to be a healthy beverage product. A healthy beverage produced from pineapple that contains many nutritions and nata de coco that contain natural fibre. The aim of addition nata de coco is as emulsifier therefore the beverage obtained from this project has better quality and longer shelf life. The activity was attended by 25 Puhrejo village residents and used two steps, that are counselling and training. The result of the activity is an increase in the community’s knowledge about the potential of pineapple and the right processing of it so the nutrition did not decrease. The desire of the community to process pineapple into a variety of products also increased because that product can provide added value to pineapple. The healthy beverage product from this activity will be a superior product from UMKM of Puhrejo village
PREPARATION AND ANTIFUNGAL ACTIVITIES OF CHITOSAN FROM APPLE-SNAIL (Pila ampullacea) SHELLS AS PRESERVING AGENT FOR POSTHARVEST PODANG MANGO (Mangifera indica L.) cv Podang Ningrum, Riska Surya; Ramadani, Aisyah; Rosalina, Reny; Yustiyani, Desy; Reyzando, Rio Altha
Indonesian Journal of Applied Research Vol. 2 No. 2 (2021): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v2i2.120

Abstract

Podang mango, an endemic mango from Kediri, has a short post-harvest shelf life that accelerates by physiological factors and pathogenic fungal contamination. Chitosan reported as an active compound for coating. The apple-snail was reported to contain chitosan in its shell. The purposes of this study are to synthesis chitosan from Pila ampullacea, examine anti-fungal activities to Fusarium oxysporum and Aspergillus ochraceus, and verify the effect of chitosan coating on shelf life and quality of Podang mango. The effect of chitosan coating on fruit quality was analyzed using three treatments which were negative control, chitosan, and chitosan-gelatin with 3 replications. The parameters measured were color, texture, aroma, water content, vitamin C, and weight loss. Results showed that chitosan synthesized from P. ampullacea had 53.56 degrees of deacetylation. Antifungal activity test of chitosan 2% had the highest inhibitory activity against F.oxysporum and A.ochraceus growth with 14.46 mm and 10.33 mm inhibition zone. Coating with chitosan statistically affected fruit quality for 14 days of storage (sig <0.05). Chitosan-gelatin coating is the best treatment in reducing weight loss (8.97%), maintain aroma (sweet fragrance), texture (soft), water content (84%), and vitamin C (0.182%), while the color (reddish yellow) was more influenced by Chitosan coating. Chitosan coated with gelatin is recommended for application on Podang mangoes.