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Screening of Antiradical Activity From Some Central Sulawesi Mangroves Dewanto, Didit Kustantio; Tanod, Wendy Alexander; Finarti, Finarti; Renol, Renol
Pharmaciana Vol 8, No 1 (2018): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.941 KB) | DOI: 10.12928/pharmaciana.v8i1.8187

Abstract

Antiradicals are substances with important functions for human health. Mangrove leaves are one of potential sources of natural antiradical. The objective of this research was to identify the type and fraction of mangrove leaves extract with the highest antiradical activity. The research procedure included sampling and extraction of mangrove leaves, assay of antiradical activity (DPPH), phytochemical assay and determination of IC50 for the fraction with the highest inhibition percentage. Mangrove leave samples (Rhizophora sp., Avicennia sp. and Sonneratia sp.) used in this study were collected from the Palu Bay coastal. Results showed the highest yield of extracts was from Rhizophora sp., followed by Avicennia sp. and Sonneratia sp. Inhibition percentage was higher from dried compared to fresh mangrove leaves. Additionally, the inhibition percentages of the ethanol fraction was higher than that of the ethyl acetate and n-hexane fractions, while Sonneratia sp. gave a higher inhibition percentage than Avicennia sp. and Rhizophora sp. The ethanol fraction IC50 was determined for
AKTIVITAS ANTIMITOTIK DARI EKSTRAK KARANG LUNAK GENUS SINULARIA Tanod, Wendy Alexander; Mangindaan, Remy E. P.; Kapojos, Magie
Journal Omni-Akuatika Vol 11, No 2 (2015): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3877.915 KB) | DOI: 10.20884/1.oa.2015.11.2.38

Abstract

Soft coral genus Sinularia be a sources of terpenoid compounds that show efficacy in vitro cytotoxic test using cancer cell lines. The study looked at the morphological changes that occur in fungal mycelial growth of Pyricularia oryzae, such as curling effect indicating antimitotic activity. Four extracts of soft coral genus Sinularia have been examination and this is an primary test to obtain information about the soft coral genus Sinularia that used as sources of compound antimitotic. This research was conducted in several stages, ie extraction of bioactive substances, P. oryzae fungal culture test, and biological testing antimitotic activity with a qualitative assessment methods. From the examination the Sinularia 3 ethyl acetate fraction show the strongest antimitotic activity (to concentration 1,5 μg/ml). Ethyl acetate fraction was purified using column chromatography. From the examination show that fraction of ethyl acetate 1 is the best fraction because still show curling effect on concentration 0,7 μg/ml.Keywords : Antimitotic, Soft Coral, Sinularia, microtubule, Pyricularia oryzae
Screening of Antiradical Activity From Some Central Sulawesi Mangroves Dewanto, Didit Kustantio; Tanod, Wendy Alexander; Finarti, Finarti; Renol, Renol
Pharmaciana Vol 8, No 1 (2018): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.941 KB) | DOI: 10.12928/pharmaciana.v8i1.8187

Abstract

Antiradicals are substances with important functions for human health. Mangrove leaves are one of potential sources of natural antiradical. The objective of this research was to identify the type and fraction of mangrove leaves extract with the highest antiradical activity. The research procedure included sampling and extraction of mangrove leaves, assay of antiradical activity (DPPH), phytochemical assay and determination of IC50 for the fraction with the highest inhibition percentage. Mangrove leave samples (Rhizophora sp., Avicennia sp. and Sonneratia sp.) used in this study were collected from the Palu Bay coastal. Results showed the highest yield of extracts was from Rhizophora sp., followed by Avicennia sp. and Sonneratia sp. Inhibition percentage was higher from dried compared to fresh mangrove leaves. Additionally, the inhibition percentages of the ethanol fraction was higher than that of the ethyl acetate and n-hexane fractions, while Sonneratia sp. gave a higher inhibition percentage than Avicennia sp. and Rhizophora sp. The ethanol fraction IC50 was determined for
SOFT CORAL (Sinularia sp.) EXTRACTS WITH ANTIBACTERIAL ACTIVITY Tanod, Wendy Alexander; Aristawati, Anita Treisya; putra, Masteria Yunovilsa; Muliadin, Muliadin
Journal Omni-Akuatika Vol 14, No 1 (2018): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (553.859 KB) | DOI: 10.20884/1.oa.2018.14.1.375

Abstract

There is a growing need for new antibacterial agents, in particular because many antibiotics are becoming ineffective due to the emergence of resistant bacterial strains. Soft corals of the Genus Sinularia, Family Alcyoniidae, have potential as a source of terpenoid and steroid compounds with antibacterial activity. These corals may vary in external morphology (shape, colour, size).The aim of this research was to identify extracted fractions with high antibacterial activity. Sinularia sp. specimens were extracted, fractionated based on solvent polarity, and tested for antibacterial bioactivity against pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Antibacterial activity of the three fractions varied in strength. The dichloromethane fraction showed strong antibacterial activity, inhibiting S. aureus and E. coli growth at a concentration of 1 mg ml-1, while the ethyl acetate and ethanol fractions were effective at 10 mg ml-1 and 100 mg ml-1, respectively.
Monitoring the endemic ornamental fish Pterapogon kauderni in Bokan Kepulauan, Banggai marine protected area, Indonesia Ndobe, Samliok; Handoko, Kris; Wahyudi, Deddy; Yasir, Moh.; Irawati, Yulina; Tanod, Wendy Alexander; Moore, Abigail Mary
Depik Vol 9, No 1 (2020): April 2020
Publisher : Faculty of Marine and Fisheries, Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (880.877 KB) | DOI: 10.13170/depik.9.1.15363

Abstract

Abstract. The Banggai cardinalfish Pterapogon kauderni is a species of national and international conservation concern. Established in November 2019, the Banggai marine protected area (MPA) in Central Sulawesi, Indonesia covers most of the endemic range of this ornamental fish. The third repeat survey (T2 monitoring) under the National Action Plan for Banggai Cardinalfish Conservation (NAP-BCFC) was carried out in October 2019 at eight sites in the Bokan Kepulauan region within the MPA. The T2 monitoring used the standard NAP-BCFC belt transect method. Data were collected on P. kauderni abundance (by size class: recruits, juveniles, adults) and microhabitat (sea urchins, sea anemones, hard corals, and others). Data were evaluated with respect to the T0 (2017) survey and T1 (2018) monitoring at the same sites, as well as previous surveys in 2004 (2 sites) and 2012 (4 sites). The data show wide between-site variation in P. kauderni and microhabitat parameters, with one subpopulation at very high risk of extirpation. Trends included declines over time in P. kauderni, sea urchin and sea anemone abundance, with an increase since 2017 in hard coral microhabitat use by adult P. kauderni. We recommend evaluation of other P. kauderni populations in Bokan Kepulauan and specific site or zone-based actions. However, we conclude that the most urgent priority for P. kauderni conservation in Bokan Kepulauan is protection of key microhabitat through a moratorium on sea urchin and sea anemone collection in P. kauderni habitat.  Keywords: Banggai cardinalfish, endangered species, marine conservation, microhabitat, monitoring, ornamental fishery, CITES
Iptek bagi Masyarakat Kelurahan Lere dalam Pengembangan Usaha Berbahan Baku Lamale (Penaeus sp.) Tanod, Wendy Alexander; Aristawati, Anita Treisya; Wahyudi, Deddy; Adel, Yeldi S
Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 5, No 1 (2019): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.5.1.11-18

Abstract

POTENTIAL MARINE-DERIVED FUNGI ISOLATED FROM SPONGE IN PRODUCE NEW AND BENEFICIAL COMPOUNDS Tanod, Wendy Alexander; Muliadin, Muliadin; Adel, Yeldi S; Dewanto, Didit Kustantio
KAUDERNI : Journal of Fisheries, Marine and Aquatic Science Vol 2 No 1 (2020)
Publisher : Sekolah Tinggi Perikanan dan Kelautan (STPL) Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.339 KB)

Abstract

Marine organisms are a source of natural products and produce compounds with a molecular structure that is unique and useful. Thousands of new compounds have biological activitity for anticancer, antiviral, and antimicrobial. This compounds isolated from various marine organism, including from marine-derived fungi. Investigation of chemical compounds from marine-derived fungi isolated from sponge has increased steadily, indicating the important role of marine-derived fungi in the discovery of drug compounds. The sponge has produced various kinds of fungi, which have reported to provide a variety of pharmacologically active metabolites and structurally diverse. Study literature showed that many fungal genera isolated from the marine sponge dominated the genus Acremonium, Aspergillus, Penicillium, Phoma, and Fusarium. The high proportion of genera and new compounds showed that the fungi isolated from the sponge could develop.
ALT BAKTERI DAN KAPANG MIE BASAH DAGING CUMI CUMI DENGAN LAMA PENYIMPANAN BERBEDA Salanggon, Alismi M; Hanifah, Hanifah; Tanod, Wendy Alexander; Hermawan, Roni
KAUDERNI : Journal of Fisheries, Marine and Aquatic Science Vol 2 No 1 (2020)
Publisher : Sekolah Tinggi Perikanan dan Kelautan (STPL) Palu

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Abstract

Mie basah merupakan mie mentah yang direbus terlebih dahulu dengan air mendidih sebelum dipasarkan. Saat ini, mie basah yang beredar di masyarakat memiliki kandungan gizi karbohidrat yang tinggi sedangkan kandungan protein dan mineral masih rendah. Oleh karena itu, penambahan mutu gizi dari mie basah dengan cara menfortifikasi mie dengan bahan lain seperti cumi cumi. Cumi cumi selain dagingnya yang mudah dicerna, juga memiliki kandungan asam amino esensial dan kaya akan mineral seperti fosfor dan kalsium yang berfungsi untuk pertumbuhan dan pembentukan tulang. Oleh karena itu, penambahan cumi cumi perlu dilakukan guna untuk meningkatkan mutu gizi dari mie. Umumnya mie basah yang beredar di pasaran hanya mampu bertahan selama ±2-3 hari pada suhu ruang. Sedangkan cumi cumi mengandung kadar air yang cukup tinggi. Kadar air yang cukup tinggi pada suatu bahan pangan, dapat memicu tumbuhnya mikroorganisme. Tujuan penelitian ini dilakukan untuk mengetahui ALT bakteri dan ALT kapang mie basah daging cumi cumi pada lama penyimpanan yang berbeda. Penelitian ini menggunakan 4 (empat) perlakuan dan ulangan sebanyak 3 kali yaitu lama penyimpanan 24, 48, 72, dan 96 jam. Hasil yang diperoleh ALT bakteri , ALT kapang dan pH pada mie basah dapat bertahan pada 48 jam pada suhu ruang sedangkan ALT bakteri, kapang dan pH pada 72 jam mie dikatakan rusak karena sudah melewati batas SNI ALT bakteri sebesar 1 x 106koloni/g dan ALT kapang sebesar 1,0 x 104koloni/g.
KANDUNGAN ANTIOKSIDAN ALGA MERAH EUCHEUMA COTTONII DENGAN METODE PENGERINGAN YANG BERBEDA Amelia, Resky; Tanod, Wendy Alexander
KAUDERNI : Journal of Fisheries, Marine and Aquatic Science Vol 1 No 1 (2016)
Publisher : Sekolah Tinggi Perikanan dan Kelautan (STPL) Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.664 KB)

Abstract

The antioxidant compound is one compound that is much sought after by researchers. Antioxidant compounds could counteract free radicals easily react with molecules such as proteins, fats, carbohydrates, and DNA. Red algae Eucheuma cottonii containing bioactive potential as natural antioxidants. Drying algae by farmers generally utilize solar energy. Drying in this way can damage the content of antioxidant compounds. This study aims to assess the proper drying method in order to minimize damage to the E. cottonii antioxidant compounds. This research was using four different drying methods of treatment and 3 replicates, in the determination of IC50 values. The yield showed dried by the sun of 70.9%, dried at room temperature with the aid of a fan at 83.3%, is dried using an oven with atemperature of 70-800C for 87.8% and dried using an oven with a temperature of 50- 600C by 73%. IC50 values in the dried by the sun at 693.90 ppm, dried at room temperature with the aid of a fan at 593.19 ppm, dried using an oven with a temperature of 70-800C at 232.87 ppm and dried in an oven at a temperature of 50-600C 419.76 ppm. Levels of antioxidants found in E. cottonii either dried using an oven or dried by the sun and the temperature of the room, including the antioxidant weak.
TINGKAT PENERIMAAN KONSUMEN TERHADAP BAKSO IKAN LELE DENGAN KONSENTRASI DAGING YANG BERBEDA Musdalifah, Musdalifah; Tanod, Wendy Alexander
KAUDERNI : Journal of Fisheries, Marine and Aquatic Science Vol 1 No 1 (2016)
Publisher : Sekolah Tinggi Perikanan dan Kelautan (STPL) Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.207 KB)

Abstract

Increased production of catfish in Central Sulawesi was not followed by an increase in the consumption of meat catfish. Diversification of products made from meat catfish, into attractive products such as meatballs need to be done. This study aims to determine consumer acceptance of meatball use catfish meat. This study uses 4 treatments and 3 repetitions. The parameters tested in each treatment, the ash content, moisture content and organoleptic assessment. The treatments were given, namely variations in the concentration of meat catfish 200 g, 400 g, 600 g and 800 g. The results showed the ash content meatballs catfish ranging from 0.64% to 0.93%. The water content meatball catfish of each treatment ranged between 66.75% - 70%. Value organoleptic appearance meatballs catfish range of 6.33 to 6.83. Smell value of meatballs catfish ranging from 6.50 to 6.83. Flavor value of meatballs catfish ranging from 6.33 to 6.83. Texture value of meatballs catfish ranging from 6.33 to 6.83. From the results of this study concluded that the levels of ash and water content meatball catfish from each treatment were given still meet the Indonesian National Standard and meatballs catfish with meat concentrations of 600 g and 800 g preferably panelists