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Current Research on Biosciences and Biotechnology
Published by Institut Teknologi Bandung
ISSN : -     EISSN : 26861623     DOI : https://doi.org/10.5614/crbb
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology
The Current Research on Biosciences and Biotechnology (CRBB) encourages researchers to publish works related to the use of the biosciences and biotechnology in understanding the world around us. From Health and Medicine to Advanced Materials, these state-of-the-art research offer novel insights in a multidisciplinary environment. We encourage scientists to submit papers focusing on the following broad topics: Health and Medicine Pharmaceutical Sciences Pharmacology and Toxicology Food Microbiology and Biotechnology Biotechnology Agriculture Bio-Energy Marine Sciences Advanced Materials Environmental Sciences
Arjuna Subject : Umum - Umum
Articles 28 Documents
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Antioxidant activity and cytotoxicity property of extracts from various coastal plants against HepG2 cell lines Vyn, Leong Kai; Kassim, Murni Nur Islamiah; Syamsumir, Desy Fitrya; Amir, Hermansyah; Ismiyarto; Andriani, Yosie
Current Research on Bioscences and Biotechnology Vol. 1 No. 2 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/10.5614/crbb.2019.1.2/ONXN6178

Abstract

Reports have shown an upward trend for liver cancer in recent years. It is also one of the deadliest cancers globally due to its complexity in detection and treatment. Hence, there is an urgency todevelop the anti-liver cancer agent from natural resources which is highly effective with minimum side effects. This study aimed to evaluate the antioxidant activity and cytotoxicity property of extracts with different polarity solvents (hexane and methanol) obtained from coastal plants collected from Merang, Terengganu. Three species (Melaleuca leucadendra, Terminalia catappa, and Rhodomyrtus tomentosa) were chosen due to their abundance and lacking anticancer studies performed on them. Green leaves were collected directly from the trees and extracted using hexane and methanol successively. Preliminary phytochemical tests (phenolic, flavonoid, terpenoid, saponin, alkaloid and glycoside) were performed on the extracts, followed by an antioxidant test based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Methanol extracts with high antioxidant property were continued for cytotoxicity study on HepG2 cell lines by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that all methanol extracts from all plants showed high antioxidant property (IC50: 0.83-1.62 mg/ml) and moderately cytotoxic activity (IC50: 24.7-28 μg/ml) against HepG2 cell lines. In contrast, hexane extracts showed very weak antioxidant activities. The highest activity was obtained by methanol extract of M. leucadendra (MLM), followed by T. catappa (TCM) and R. tomentosa (RTM), respectively. These promising results indicated that MLM could be a potential candidate for further study related to the antioxidant and cytotoxicity on HepG2 cell lines, such as for anti-liver cancer agent.
Iron absorption stimulation by administration of soy protein hydrolysates containing bioactive peptides in rats Susanti, Ida; Laily, Noer; Aji, Galih Kusuma; Muhamaludin; Ikhsan, Haris Muhamad
Current Research on Bioscences and Biotechnology Vol. 1 No. 2 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/10.5614/crbb.2019.1.2/DKPB8204

Abstract

The prevalence of iron deficiency anemia in Indonesia is high. Most of the anemia is caused by iron deficiency syndrome due to inadequate iron intake and low bioavailability of iron sources. Some studies indicated that bioactive peptides from certain protein hydrolysates could support iron absorption. Our previous study showed that soy protein hydrolysates containing bioactive peptides indicated an iron-binding activity and could increase its solubility in water. Aim of this study was to investigate the effect of soy protein hydrolysates administration on iron serum levels in anemic Sprague Dawley rats. Anemia induction was performed by applying NaNO2 (0.5 mg/ml per 200 g body weight (BW) of rats per day for 14 days) orally. The experiment was carried out in a completely randomized design, consisting of six groups. The first group was the control normal group (N), without induction and the others were treatment groups that were induced with NaNO2 and supplemented with iron (0.3 mg/200 g BW), consisted of control group (CMC), control iron (Fe) group, iron and soy protein hydrolysate-1 (270 mg/200 g BW) (FeSH-1) group, iron and soy protein hydrolysate-2 (270 mg/200 g BW) (Fe-SH2) group and positive control iron vitamin C (0.24 mg/200 g BW) (Fe Vit. C) group. The result showed that Fe-SH2 treatment group had a better iron-binding capacity rather than other groups. This result suggests that soy protein hydrolysates could enhance iron absorption, which might be applied in human as a functional food to reduce anemia prevalence.
Partial properties of ready-to-use shrimp paste affected by heating time Handayani, Baiq Rien; Dipokusumo, Bambang; Werdiningsih, Wiharyani; Rahmadhina, Sitta Fitri
Current Research on Bioscences and Biotechnology Vol. 1 No. 2 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/10.5614/crbb.2019.1.2/JTHM4041

Abstract

Ready-to-use shrimp paste is a traditional shrimp paste produced by a small producer scale in East Lombok through the non-fermentation process and continued by un-control heating time. The qualities were ununiform. Aim of this study was to determine the physical, sensory and microbial quality of ready-to-use shrimp paste affected by heating time. The method conducted was a completely randomized design. The heating time was arranged for 0 to 70 min, with a 10 min interval. The data was analyzed using Co-Stat Software at 95% significant level, then further tested for significance difference data with Tukey’s HSD. Microbial data was analyzed using descriptive method. The result showed that the properties of ready-to-use shrimp paste were influenced by heating time of controlled oven. The heating time affected moisture content, oHue, sensory of aroma, taste and texture. However, it did not affect the appearance and L value (brightness). Heating time for 50 min at 100oC using the controlled oven is recommended as the best treatment to produce ready-to-use shrimp paste with 32.61% of moisture content, 3.9 x 103 CFU/g of total microbe, less than 102 CFU/g of total fungi and undetected coliform. The shrimp paste had yellow-red color, clean appearance which was specific shrimp paste, very specific odor and taste. It also has a compact texture. Heating time for 50 min at 100oC can extend the shelf life of ready-to-use shrimp paste up to minimum 60 days at room temperature.
Nephroprotective effect of extract Etlingera elatior (Jack) R.M. Smith on CCl4-induced nephrotoxicity in rats Fristiohady, Adryan; Wahyuni, Wahyuni; Y, Muhammad Ilyas; Bafadal, Mentarry; Purnama, La Ode Muhammad Julian; Sangadji, Firasmi; Malaka, Muhammad Hajrul; Malik, Fadhliyah; Hong, Junli; Yodha, Agung Wibawa Mahatva; Sahidin, I
Current Research on Bioscences and Biotechnology Vol. 1 No. 2 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/10.5614/crbb.2019.1.2/DAPM6106

Abstract

Reactive oxidative stress (ROS) can lead to cell damage, and one of them is the kidney's cell. Etlingera elatior (Jack) R.M. Smith can be utilized as an agent that can protect the cell from ROS. This study aimed to investigate the protective effect of E. elatior fruit on the kidney's cell. We used experimental animals which were treated with Na-CMC (Group I), Na-CMC (Group II), the extracts of E. elatior fruit 200, 300, 400 mg/kg BW for Group III, IV, and V, respectively, for seven days. The blood was collected after treatment. At day 8, group I, III, IV, and V were induced by CCl4. At day 9, blood was collected and the kidneys were harvested for histopathology analysis. Blood collected were measured for albumin, total protein, urea and creatinine levels. After treatment, albumin and total protein showed no increased levels; urea decreased at doses of 200, 300, and 400 mg/kg BW, respectively, and creatinine levels only decreased at the dose of 400 mg/kg BW (p<0.05). The dose of 200 and 300 mg/kg BW showed protecting effects in the tubular cells of renal. Therefore, the ethanol extract of E. elatior showed a nephroprotective effect by normalizing the urea and creatinine levels of rats and protecting tubular cells of renal.
The effect of packaging materials on the physicochemical stability of ground roasted coffee Agustini, Sri; Yusya, Mimi Kurnia
Current Research on Bioscences and Biotechnology Vol. 1 No. 2 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/10.5614/crbb.2019.1.2/ZTVC3720

Abstract

This research was intended to evaluate the effect of packaging materials on the stability of physicochemical properties of ground roasted coffee during storage at room temperature (28±2°C) and relative humidity (RH) 80% for 12 months. The packaging material used was aluminium-laminated polyethylene (ALP) and polyethylene terephthalate (PET). The physicochemical characteristics observed were non-volatile fractions consisted of moisture, crude fibre, ashes, the alkalinity of ashes, pH, microbiological load, total phenolic and caffeine contents. The results showed that the moisture content of ground coffee was below 5.0 % for both packaging materials. The overall adsorbed moisture of ground coffee was significantly different (p<0.05) for both packaging materials, whereby the moisture content of ground roasted coffee packaged in PET was 4.15% while of those packaged in ALP was 3.38% by the end of 12 months storage. There was no significant decrease in the ashes, the alkalinity of ashes, crude fibre, pH, total phenolic and caffeine contents for both packaging materials. The total plate count (TPC) decreased from an initial of 1.2 x 103 to be 1.7 x 102 CFU/g. Both packed ground roasted coffees were microbiologically safe as it had TPC less than 105 CFU/g after 12 months storage. The results indicated that ALP and PET packaging can preserve the quality and stability of the ground coffee during storage.
Removal of sulfur and ash from Indonesian coal by indigenous mixotrophic bacteria Arifin, Mubdiana; Sanwani, Edy; Chaerun, Siti Khodijah
Current Research on Bioscences and Biotechnology Vol. 1 No. 2 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/10.5614/crbb.2019.1.2/QNZG5857

Abstract

Coal is one of the alternative fuels which potentially provides most of the domestic energy needs. The steam-powered electric generator (PLTU) is a sector that dominates the utilization of domestic coal. Sulfur contained in the coal is an element impurity apart from other contaminants such as ash, soil, rocks, and minerals. The combustion of high-sulfur from coal produces SO 2 which can interfere the human health, such as causing tightness in the respiratory tract, as well as the environment by causing acid rain and corrosion on plant equipment. Various efforts have been made by reducing the levels of sulfur to minimize the negative impact caused by coal combustion. The utilization of bacteria for biodesulfurization has been developed and widely studied as an alternative treatment to remove the sulfur and ash from the coal. Therefore, the purpose of this study was to remove the sulfur and ash from coal using various types of indigenous bacteria by the biomining method. The current study used coal from East Kalimantan of Indonesia with a total sulfur and ash content of 2.56 and 7.21%, respectively. The indigenous bacteria used in this study consisted of five bacterial isolates identified as Citrobacter murliniae, Dietzia psychralcaliphila, Pseudomonas aeruginosa, Alcaligenes faecalis, Bacillus altitudinis. The results showed that the bacterium C. murliniae was able to eliminate the sulfur by 19.61%, which was higher than the other bacterial isolates and remove ash from coal by 1.75%. The bacteria D. psychralcaliphila, P. aeruginosa, A. faecalis, B. altitudinis were capable of eliminating 16.49, 8.30, 3.61, 8.89% of total sulfur and 4.03, 4.56, 5.29, 4.21 of ash content in coal, respectively.
Antibacterial and antioxidant potentials of methanol extract and secondary metabolites from Wualae rhizome (Etlingera elatior) Wahyuni; Grashella, Sri Hutami Lety; Fitriah, Wa Ode Ida; Malaka, Muhammad Hajrul; Fristiohady, Adryan; Imran; Sahidin
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The rhizome of Etlingera elatior or Wualae (Tolakinese) has many advantages on traditional remedies and cooking in Sulawesi Tenggara. To support those advantages, two secondary metabolites derived from steroid and phenylpropanoic acid classes, stigmast-4-en-6β-ol-3-one (1) and p-coumaric acid (2), respectively, have been firstly isolated and identified from the E. elatior rhizome. Isolation of these two compounds was performed using several chromatography techniques, including thin layer chromatography (TLC), vacuum liquid chromatography (VLC) and radial chromatography (RC). Identification of isolates was carried out using 1 H and 13C NMR spectroscopy and comparing the spectroscopy data with the library. The potency of antibacterial of the methanol extract of Wualae rhizome and the isolates were evaluated against Pseudomonas aeruginosa ATCC 27853, Bacillus subtilis FNCC 0060, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Salmonella enterica ATCC 14028, and Streptococcus mutans ATCC 25175 using agar diffusion method. Antioxidant activity was evaluated against DPPH radicals (2,2-diphenyl-1-picrylhydrazyl). The results show that the antibacterial potential of Wualae methanol extract is better than compound 1 and 2. Furthermore, the antioxidant properties of Compound 2 (IC50 159.47 μg/mL) was stronger than the antioxidant properties of Compound 1 (IC50 219.95 μg/mL) and the methanol extract (IC50 586.38 μg/mL).
The tremendous contribution of biosciences and biotechnology for better life: research dissemination is one of the most important role to make it happen Elfahmi
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

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Abstract

Biosciences and biotechnology have become the interesting topics since long to be investigated in order to understand science and technology, develop many tools and instrumentation, discovery of new drugs including natural base drugs and biopharmaceutical, contribute in energy sources, interact with the environment as well as to improve the quality of human being. The researches of the field have dramatically contributed for many other field and for better life. Uncounted numbers publications in these field will be continuously rising. To contribute in dissemination of research output which have been done by researcher around the world, the Current Research on Biosciences and Biotechnology so called CRBB is published in this year. In the beginning this journal will publish 2 issue in the year and will be increased in the future. All researchers in the field of bioscience and biotechnology are invited to submit the manuscripts to CRBB. The manuscript will be peer reviewed prior to publish.
Revisiting pollen-pistil interaction and cross incompatibility in maize Nugrahapraja, Husna; Bertolini, Edoardo; Pe, Mario Enrico
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

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Abstract

The review addressed aspects of plant fertilisation and the phenomenon of genetic cross-incompatibility in maize controlled by the Gametophyte1 locus. This phenomenon determines the failure to accomplish successful fertilisation and a full seed set when pollen grains carrying the ga1 allele pollinate female inflorescences carrying the Ga1-strong (Ga-1s) allele in the homozygous state (Ga1-s/Ga1-s). We divided the review work into several topics — first, the introduction of sexual plant reproduction. Second, pollen-pistil interactions in plants. Third, reproductive barriers during plant reproduction. Third, Incompatibility in plants. Fourth, fine mapping of the Ga1 locus in maize. Fifth, recent researches on Ga1-related cross-incompatibility in maize.
Effect of tree age on the yield, productivity, and chemical composition of essential oil from Cinnamomum burmannii Fajar, Anugerah; Ammar, Ganjar Abdillah; Hamzah, Muhammad; Manurung, Robert; Abduh, Muhammad Yusuf
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

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Abstract

One of the factors that influence the yield of cassia oil is the age of tree. Cassia bark is normally harvested at a tree age of 5 years old and continue to be harvested until 15 years of age. This study investigated the effect of tree age of Cinnamomum burmannii (5, 12 and 20 years old) on the yield, productivity and chemical composition of essential oil from leaf, branch and trunk bark. The essential oil was extracted using steam distillation and liquid-liquid extraction methods. The results showed that the optimum yield of Cinnamomum burmannii oil was obtained when the water content was in the range of 36-47%. The optimum yield of essential oils from the leaf was obtained at 1.36±0.31 wt% (5 years old) and for the branch and the trunk bark were obtained at 3.2±0.07 wt% and 2.95±0.30 wt% (both were 12 years old). Chemical composition of the essential oil was also analysed. The major components of Cinnamomum burmannii oil were determined as cinnamaldehyde (68.3%-82%), cinnamyl acetate (2.5%-16%), cinnamyl alcohol (2.25%-4.6%), and cinnamic acid (3%-8%). The productivity of essential oil was estimated at 336 kg/ha.year (5 years old), 577 kg/ha.year (12 years old) and 387 kg/ha.year (20 years old).

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