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Titania T Nugroho
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INDONESIA
Jurnal Natur Indonesia
Published by Universitas Riau
ISSN : 14109379     EISSN : 25030345     DOI : http://dx.doi.org/10.31258/jnat
Core Subject : Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry
JURNAL NATUR INDONESIA terbit sejak tahun 1998, merupakan jurnal ilmu sains yang menyajikan artikel mengenai hasil penelitian, pemikiran dan pandangan dari peneliti dan pakar dalam bidang biosains (ilmu dasar), meliputi biologi, fisika, kimia dan matematika. Jurnal Natur Indonesia melibatkan mitra bestari yang menelaah setiap artikel sesuai dengan bidang ilmunya. Nama dan asal institusi mitra bestari tersebut tercantum pada halaman bagian normor 2 dari setiap volume penerbitan. Jurnal ini diterbitkan setahun dua kali, pada bulan April dan Oktober.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 195 Documents
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Synthesis, Characterization and Anti Mcf-7 Proliferative Acitivity of Flavonol Derivatives of 2'-Hydroxyacetophenon and 3,4,5-Dimethoxy Benzaldehid Rokim, Muhamad; Zamri, Adel; Teruna, Hilwan Yuda
Jurnal Natur Indonesia Vol 17, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.257 KB) | DOI: 10.31258/jnat.17.2.16-24

Abstract

Flavonols a derivatives of 2'-hydroxyacetophenon and 3,4,5-dimethoxy benzaldehid has been synthesized under basic condition (KOH). The structure of the compound was characterized based on the interpretation of spectroscopic data, including UV, FTIR, NMR and HRMS. Anticancer activity was evaluated using the MTS assay against MCF-7 cells, which showed that the flavonol 2'-hydroxycalone derivative was potentially active as an anticancer substance with IC50 values <1000 µg / mL.
Efek Sumber Karbon Berbeda terhadap Produksi â-Glukan oleh Saccharomyces Cerevisiae pada Fermentor Air Lift Kusmiati, Kusmiati; Thontowi, Ahmad; Nuswantara, Sukma
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.458 KB) | DOI: 10.31258/jnat.13.2.138-145

Abstract

The need of â-glucan is increasing in food, medicine and cosmetic industry, because it becomes anticancer,antitumor and antiaging, increases immunosystem, and decreases cholesterol content in blood. The cell walls ofS. cerevisiae contain 80-90% polysaccharides that posses â-glucan. This research was aimed to obtain appropriatecarbon sources to increase the production of â-glucan. The carbon sources used were glucose, glucose commercial,sucrose and molases. The fermentation process was done by using air lift fermentor. The steps of fermentatonincluded regeneration of S. cerevisiae strain, preculture, fermentor preparation and running fermentor for 84hours. Sampling of S. cerevisiae culture was determined the cell growth by optical density (OD) usingspectrophotometer UV/VIS at ë 550 nm. The protein content was determined by Lowry method at ë 755 nm and thetotal glucose was measured by phenol sulphate method at ë 490 nm. The measurement result of cell growthshowed that the high intensity of S. cerevisiae in medium contain molases, but it did not show significant effectwhen compare to other carbon sources. The protein and carbohydrate contain in medium tended to decrease. Theresult of â-glucan on glucose, sucrose, glucose commercial and molases were 933,3, 1100, 1000, and966,7 mg/l. It can be concluded that sucrose and glucose commercial can replace the glucose to produce of â-glucan, because they are cheaper and easier to get. Beside that, molases can be used as an alternative carbonsource because it can produce of â-glucan as well as glucose.
Kajian Kondisi Terumbu Karang dan Strategi Pengelolaannya di Pulau Panjang, Air Bangis, Kabupaten Pasaman Barat Oktarina, Angreini; Kamal, Eni; Suparno, Suparno
Jurnal Natur Indonesia Vol 16, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2639.219 KB) | DOI: 10.31258/jnat.16.1.23-31

Abstract

Coral reef ecosystems are essential for the survival of marine life, but the presence of coral reef are declined and decayed.Methods used in this study was a survey method. To retrieve the condition of coral cover using the line intercept transectwhile to determine management strategies using SWOT analysis. The average percentage of coral cover in 8 point researchstations at 50.10% are still in the good condition, which is in the north 3 m depth 53.51%, north 7 m depth 68.14%, east 3 mdepth 93.29%, east 7 m depth 15.93%, south 3 m depth 75.17%, south 7 m depth 20.53%, west 3 m depth 41.50%, and west7 m depth 32.79%. The strategies for coral reef ecosystem management, among others: 1) community-based management, 2)forming Kelompok Masyarakat Pengawas (Pokmaswas) institutions, 3) establish in Panjang Island as a marine conservationareas, 4) Ko-Management-based, 5) effort to improve the economy in order to support the increased knowledge of theimportance of conservation and management of coral reef ecosystem.
Isolasi dan Karakterisasi Protease Ekstraseluler dari Bakteri dalam Limbah Cair Tahu Fatoni, Amin; Zusfahair, Zusfahair; Lestari, Puji
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.484 KB) | DOI: 10.31258/jnat.10.2.83-88

Abstract

Protease has been used in large application industrial process such as detergent, leather, textil, softdrink, andmedicine. In order to find unique protease, many substances were explored as proteases of bacteria sources. Inthis study, tofu liquid waste was used as a source of bacteria producing proteases. Waste sample was growth inskim milk agar medium showing proteases activity, it was used to produce extracellular protease. The microbialcolonies were identified as Staphyllococcus sp. Protease was extracted with 5000 g centrifugation at 4 0C, andpurificated with ammonium sulphate precipitation continued with dialisis. Optimum production time, pH, metal ion,EDTA, specific activity, KM, and Vmaks were studied for enzyme characterization. Volume of crude enzyme was 300ml, with spesific activity of 3.55 U/mg. Protease obtained from 60% ammonium sulphate fraction had the highestspecific activity of 68.22 U/mg. Study on the protease characterization revealed that optimum temperature of thisenzyme was 400C. The optimum pH of the enzyme was found to be 8.0. The kinetic parameters K M dan Vmaks withcasein as substrate were 0.31% and 51.55 U/ml. Some inhibitory effect was observed in the presence of EDTA, Cu +2,Co+2, Zn+2, and enzyme activity was stimulated by Mg+2, indicating that this ion had a functional role in the molecularstructure of the enzyme.
Penggunaan Zeolit sebagai Pendegradasi Senyawa Permetrin dengan Metoda Fotolisis Zilfa, Zilfa; Suyani, Hamzar; Safni, Safni; Jamarun, Novesar
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.086 KB) | DOI: 10.31258/jnat.14.1.14-18

Abstract

The research about the degradation of permethryn compound has been done by photolysis method using natural zeolyte as catalyst.Permethryn is one syntetic pyretroid pesticides that low toxicity for mammals but it is high toxicity for fishs, insects and water microorganisms.Zeolyte is aluminosilicate minerals that can be used for degradation of permethryn. Percentage degradation of permethryn is lower withoutzeolyte than using zeolyte as catalyst by photolysis method. 20 mg/l permethryn solution could be degraded 5.40% after 120 minutesirradiation, while by adding 0.20 g natural zeolyte, permethryn could be degraded 69.70
Studi Nutrisi Buah Ara (Ficus racemosa L.) untuk Pakan Ikan Aryani, Netti; Zen, Zuhelmi; Syandri, Hafrijal; Jaswandi, Jaswandi
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.384 KB) | DOI: 10.31258/jnat.12.1.54-60

Abstract

The purpose of the research was to find out the countent of nutrition of Ara (Ficus racemosa L) wich covers : thelevel of protein, fat, water, ash, fibrous part of the fruit, calcium, phosphor, vitamine C and E as well as amino acidwhich are very useful for enrichment of fish diets. The sample of Ara fruit were collected directly in the field. Therewere two types of processing Ara fruits used in this research, namely (1) cutting the fruits into thin layer and madeit into; (2) separating the fuit from the seed, dried, and made it into flour. The level of protein, fat, fibrous part of thefruit, water, and ash was analyzed by using proximate analysis, while the level of calcium was analyzed by usingAAS methods. The level of phosphor was measured by using spectrophotometer, the level of vitamine C and E wasanalyzed by using HPLC and amino acid by gas chromatography (CG). The study found that the flesh flour of Arafruit contained protein (10,63%), fat (8,66%), water (7,08 %), ash (11,57%), fibrous part of the fruit (16,15%), calcium514,88 mg/100 g), phosphor (418,29 mg/100 g), vitamine C (475,38 mg/100 g), and amino acid (3,013 %) higher thanAra flour with protein (8,98 %), fat (6,57%), water (10,26%), ash (18,23%), fibrous part of the fruit (20,31%), calcium(0,039 mg/100g), phosphor (0,002 mg/100g), vitamin C 25,48 mg/100 g ), and amino acid (1,24%). It can be concludedthat the Ara flour can be used as on of the ingredients for fish diets especially as the source of vitamine C, calciumand phosphor to incrase reproduction ability and growth.
Sintesis Tiga Peptida Bergugus Pelindung sebagai Prekursor Komponen Vaksin Influenza Universal Subroto, Toto; Hardianto, Ari; Kahari, Abdul Alim; Pradnjaparamita, Tika
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.702 KB) | DOI: 10.31258/jnat.15.2.84-91

Abstract

Current highly effective conventional vaccine to halt the spread of bird flu has not been invented yet because of susceptiblemutation of influenza virus. In spite of undergoing mutation which causes the amino acid sequence change, influenzaviruses maintain conservation at ectodomain of M2 protein, especially M2e(2-16) (SLLTEVETPIRNEW). The use ofconserved epitope M2e(2-16) in epitope-based vaccine potentially produces universal influenza vaccine. In designingepitope-based vaccine, the M2e(2-16) needs to be coupled with T helper epitope, P25, which is subsequently mentioned asM2e(2-16)-K-P25 (SLLTEVETPIRNEWGKKKL IPNASLIENCTKAEL). The M2e(2-16)-K-P25 was synthesized usingconvergent solid phase peptide synthesis strategy because of the size of the sequence. In this strategy, four peptideprecursors of M2e(2-16)-K-P25; SLLTEVETP (F1), IRNEWGK (F2), KLIPNASLI (F3), and ENCTKAEL (F4); were synthesizedin advance. After the precursors ready, coupling reaction was performed to obtain M2e(2-16)-K-P25. In the previousresearch, F3 has been obtained in high purity through Fmoc/tBu solid phase peptide synthesis method. In this conductedresearch, the three remaining precursors; F1, F2, and F4; were synthesized by the same method. Each peptide was analysedby thin layer chromatography, HPLC, and mass spectroscopy methods. F1, F2 and F4 were successfully synthesized andeach of them was detected at 1490.0, 1874.8 and 1881.9 amu, respectively. However, F1 was not possible to purify becauseof its insolubility in various solvents.
Uji Efek Pelindung Hati Ekstrak Air Tanaman Kulit Lawang, Cinnamomum Cullilaban (Lauraceae), melalui Pemeriksaan MDA dan SOD Plasma Sel Darah Tikus Soeksmanto, Arif
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.103 KB) | DOI: 10.31258/jnat.13.2.130-133

Abstract

Industrial growth, pollution, and the change of life style cause environmental degradation. This condition enhancesthe occurrence of degenerative diseases that more difficult to conquer than infective diseases. This promotes theuse of herbs in America up to 385% in period 1990-1997, wasting 4–12 billion dollar each year. World HealthOrganization (WHO) estimates approximately 4 billion or 80% of world citizens use herbs for their health care. Kulitlawang plants are medicinal plants grown endemically in Papua and Maluku regions. The existence of the plants inMaluku islands is considered as endangered species. Information on the plants in scientific publication is relativelylimited. meanwhile Cinnamomum sp. is known having high antioxidant compound. This research aimed to find outhepatoprotector effect of kulit lawang water extract through observation of MDA and SOD of venous blood of ratintoxicated with CCl4. Dosage used was based on result of effective dosage test which showed that water extractof kulit lawang plant of 20 mg/kg bw as highest dosage that did not harm animal health. Result of hepatoprotectoreffect test showed that MDA and SOD of control were 230.12 U/ml and 1243.00 ρmol/ml, whereas water extract ofkulit lawang was 414.45 U/ml and 793.00 ρ mol/ml, á-tocopherol 416.42 U/ml and 782.33 ρmol/ml, and curcumin426.07 U/ml and 734.00 ρmol/ml. This showed that water extract of kulit lawang plant has antioxidant propertiesand potential as hepatoprotector of oxidative damage.
Efek Pembukaan Lahan terhadap Karakteristik Biofisik Gambut pada Perkebunan Kelapa Sawit di Kabupaten Bengkalis Suwondo, Suwondo; Sabiham, Supiandi; Sumardjo, Sumardjo; Paramudya, Bambang
Jurnal Natur Indonesia Vol 14, No 1 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (968.06 KB) | DOI: 10.31258/jnat.14.1.143-149

Abstract

Oil palm plantations at peatland are faced with problems of land degraded, low productivity and produced to green house gas (GRG) emission.The objectives of this study were to land clearing effect of peatlands and to identified factors that affect the peatlands in the biophysicalcharacteristics. This study was conducted on District Bengkalis-Riau at marine and brakish peat. The data was collected using field survey anddocumentation. The descriptive analysis was used to determine biophysical characteristics and linier regresion was used to corelation performimportant factors. The research results showed that the biophysical characteristics of peatland experienced changes in the horizon profile,peat thickness, decomposition level, moisture content, ash content, pH, C-organic and biomass. The secondary peat swamp forest ofbiophysical characteristics have to different in the horizon profile, peat thickness, decomposition level, moisture content, ash content, pH,C-organic and biomass compared with marine and brackish peat.
Pola Resistensi Bakteri Penyebab Infeksi Saluran Kemih (ISK) Terhadap Antibakteri di Pekanbaru Endriani, Rita; Andrini, Fauzia; Alfina, Dona
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.146 KB) | DOI: 10.31258/jnat.12.2.130-135

Abstract

Urinary Tract Infection (UTI) is caused by a variety of microorganism, but bacteries are more often. Antibacterial isthe empiric treatment for UTI. The antibacterial resistance pattern of UTI can be changed by the place and time.Urine culture test and sensitivity test will help us to choose the effective treatment. The purpose of this researchis to find out the pattern of UTI in Pekanbaru. Results of this research showed that UTI bacterial resistancy mostlyfound in Gram negative bacateria with penicillin and 1 st cephalosporin antibiotic.

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