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Contact Name
Kurniatun Karomah
Contact Email
hsji.indonesia@gmail.com
Phone
+6281287852886
Journal Mail Official
hsji.indonesia@gmail.com
Editorial Address
Jl. Percetakan Negara no. 29 Jakarta Pusat
Location
Kota adm. jakarta pusat,
Dki jakarta
INDONESIA
Health Science Journal of Indonesia
ISSN : 20877021     EISSN : 23383437     DOI : https://doi.org/10.22435/hsji
Core Subject : Health,
Health Science Journal of Indonesia is a journal developed to disseminate and discuss the scientific literature and other research on the development of health. This journal is intended as a medium for communication among stake holders on health research such as researchers, educators, students, practitioners of Health Office, Department of Health, Public Health Service center, as well as the general public who have an interest in the matter. The journal is trying to meet the growing need to study health. Vision: Becoming a notable national journal in the field of health research and towards a reputable international journal. Mission: Providing scientific communication media in health research in order to advance science and technology in related fields. Organizes scholarly journal publishing in health research with an attempt to achieve a high impact factor in the development of science and technology.
Articles 77 Documents
Back Matter HSJI Volume 11 no. 2 2020 author, hsji
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

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Back Matter HSJI Volume 11 no.2 2020
Back Matter HSJI Volume 11 no. 1 2020 author, hsji
Health Science Journal of Indonesia Vol 11 No 1 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

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Back Matter HSJI Volume 11 no. 1 2020
Front Matter HSJI Volume 11 no.2 2020 author, hsji
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

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Front Matter HSJi Volume 11 no.2 2020
Laboratory preparedness to support the Covid-19 pandemic respond in Indonesia Aryastami, Ketut; Hendarwan, Harimat; Setiawaty, Vivi; Su'udi, Amir; Mulyani, Ully Adhie; Susilawati, Made Dewi; Syachroni, Syachroni; Puspandari, Nelly; Suwandono, Agus
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.4089

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Latar belakang: Penyakit jenis baru COVID-19 yang disebabkan oleh virus corona menjadi sebuah pandemic di akhir tahun 2019. Kota Wuhan (China) merupakan lokasi pertama terdeteksinya kasus COVID-19. Tanpa adanya kecurigaan apapun penyakit ini dengan cepatnya menyebar ke seluruh dunia mengikuti alur mobilitas manusia. Dalam kondisi tersebut sistem kesehatan di setiap negara tampak kelabakan khususnya dalam pengendalian transmisi penyakit. Studi ini ingin mengidentifikasi kesiapan jejaring laboratorium kesehatan di Indonesia. Metode: Penilaian cepat dilakukan terhadap ketersediaan dan kesiapan laboratoriaum dalam pennanganan pandemi Covid-19. Pengumpulan data dilakukan melalui pengisian questioner yang dikirim secara elektronik. Waktu pelaksanaan adalah minggu ketiga dan keempat, Maret 2020. Terdapat 44 laboratorium jejaring laboratorium dibawah Kementerian Kesehatan yang menjadi subjek penelitian, dan sebanyak 33 yang merespon secara lengkap Variabel ketersediaan, kecukupan dan kebutuhan bahan dan alat. Hasil: Jejaring laboratorium kesehatan dibawah Kementerian Kesehatan sudah terbentuk sejak tahun 2009. Dengan terjadinya pandemic COVID-19 Surat Keputusan Menteri Kesehatan telah direvisi hingga dua kali agar dapat meningkatkan kapasitas dan memperluas jejaring ke seluruh wilayah NKRI. Hasil studi menunjukkan, laboratorium jejaring dibawah Kementerian Kesehatan belum siap dalam menghadapi pandemic COVID-19. Dua jenis laboratorium jejaring yaitu laboratorium surveillans maupun laboratorium diagnostic memiliki kondisi yang sama. Ketersediaan bahan dan alat laboratorium standar masih tergolong rata-rata, bahkan dari sisi kecukupannyapun masih jauh dibawah kapasitas kebutuhan dalam penanganan specimen COVID-19. Kondisi yang sama juga tampak untuk bahan pendukung laboratorium termasuk alat pelindung diri untuk petugas. Kesimpulan: Kesiapan laboratorium sebagai bagian dari system kesehatan dalam kondisi pandemic masih lemah. Keberadaan alat penunjang diagnose khususnya untuk penyakit menular harus dilengkapi sesuai dengann type laboratorium. Pandemi COVID-19 menjadi alarm dalam menghadapi era baru dan antisipasi masalah dimasa yang akan datang. Kata kunci: Kesiapan laboratorium, COVID-19, Indonesia Abstract Background: A novel coronavirus disease called COVID-19 has become pandemic in late 2019. Wuhan City was the first place detected as the source of the pandemic. Without suspicion, it spreads over the world, along with human mobility. In such a condition, every country seems quite stuttering to prepare its health system to prevent its people from the possible transmission. This paper aims to describe the preparedness of the networking laboratory in Indonesia. Methods: We conducted a rapid assessment of laboratory availability and preparedness to respond to the Covid-19 pandemic. We held the data collection on the third and fourth week of March 2020 by sending an electronic questionnaire to all 44 networking laboratories under the Ministry of Health structure. The variables assessed in this study were the availability and the requirements of the Covid-19 related laboratory's substances, including reagents and other equipment types. Results: The Ministry of Health established the networking laboratory in 2009, but due to the COVID-19 pandemic, it has renewed twice to enhance and expand the laboratory capacities over the country. Our studies showed preparedness among networking laboratories in Indonesia regarding this new emerging COVID-19 condition was quite devastating. Both surveillance and diagnostic laboratories have a similar situation. The availability of their primary materials was mediocre, but the adequacy was far beyond the capacity in handling the COVID-19 specimen. We found a similar case in the laboratory, supporting materials, and personal protective equipment (PPE). Conclusion: Laboratory preparedness during initial period of time of the COVID-19 pandemic as part of the health system is still weak. The availability of the necessary equipment, supporting materials, and personal protective equipment are far beyond the requirements. The COVID-19 has alarmed the laboratory and the whole health system in Indonesia into a new era with better future preparedness. Keywords: laboratory preparedness, COVID-19, Indonesia
Overexpression of MiR-155-5p and increased number of macrophage population in precancerous prostatic disease Putri, Rachma Greta; Pratiwi, Sari Eka; Heriyanto, Didik Setyo; Danarto, Danarto; Astuti, Indwiani; Arfian, Nur; Haryana, Sofia Mubarika
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.3952

Abstract

Latar Belakang: Gangguan regulasi mikroRNA(miR) dan inflamasi kronik dapat mengubah tumor menjadi karsinoma dan kanker dengan metastasis melalui perubahan seluler dan genomik. Lesi prekanker memiliki peluang 33,3 persen menjadi kanker. Penelitian ini bertujuan untuk mengkaji peran miR-155-5p terhadap mRNA SOCS1 dan populasi makrofag terhadap progresivitas penyakit yang berhubungan dengan Benign Prostate Hyperplasia (BPH), High Grade Prostatic Intraepithelial Neoplasia (HGPIN), dan Prostate Adenocarcinoma (PRAD). Metode: Penelitian ini merupakan penelitian potong lintang dengan 3 kelompok, yaitu BPH,HGPIN, dan PRAD. Sampel jaringan didapatkan dari Tindakan TURP. Ekspresi miR-155 dianalisis menggunakan qPCR dan dikalkulasi menggunakan metode Livak. Ekspresi mRNA SOCS-1 dianalisis menggunakan reverse transcriptase PCR. Penanda pan makrofag, anti CD-68 monoclonal antibody(MoAb) digunakan untuk mendeteksi populasi makrofag pada jaringan dengan imunohistokimia. Hasil: Ekspresi miR-155 lebih tinggi pada HGPIN dibandingkan BPH dan PRAD (p=0,14). Ekspresi mRNA SOCS1 pada HGPIN paling rendah diantara ketiga sampel (p=0,96). Terdapat korelasi negative antara miR-155 dan mRNA SOCS1 (p=0,02). Terdapat peningkatan persentase populasi makrofag yang signifikan pada HGPIN (6,03 persen) dibandingkan BPH (0.89 persen) dengan p=0,00. Kesimpulan: Pada penelitian ini, terdapat perubahan persentase makrofag dan miR-155 pada HGPIN. Variasi ekspresi miR-155 dan persentase populasi makrofag dapat disebabkan karena perubahan epigenetik. Oleh sebab itu, perlu penelitian lebih lanjut untuk memvalidasi hasil tersebut dan memahami kemungkinan menjadi biomarker pada penyakit prekanker pada prostat. Kata Kunci: Prostatic Intaepithelial Neoplasia, miR-155, Makrofag Abstract Background: Impaired microRNA(miR) regulation and chronic inflammation could transform tumors into carcinoma and cancer by metastasis through cellular and genomic changes. Precancerous lesions have a 33.3 percent chance of becoming cancerous. This study investigated the role of miR-155 related to SOCS1 mRNA and macrophage population in disease progression associated with Benign Prostate Hyperplasia (BPH), High-Grade Prostatic Intraepithelial Neoplasia (HGPIN), and Prostate Adenocarcinoma (PRAD). Methods: This was a cross-sectional study using three groups of samples, namely BPH, HGPIN, and PRAD. Tissue samples were obtained from TURP Action. The expression of miR-155 was analyzed using real-time qPCR and calculated using the Livak method. The expression of SOCS1 mRNA was analyzed using reverse transcriptase PCR. The macrophage pan-marker, anti-CD68 monoclonal antibody (MoAb), was used to detect macrophage population in tissues by immunohistochemistry. Results: The expression of miR-155 was higher in HGPIN than BPH and PRAD (p=0.14). The expression of SOCS1 mRNA in HGPIN was the lowest among the three samples (p=0.96). There was a negative correlation between miR-155 and SOCS1 mRNA (p=0.02). There was a significant increase in the percentage of the macrophage population in HGPIN (6.03 percent) compared to BPH (0.89 percent) with p=0.00. Conclusion: In this study, there were changes in the percentage of macrophage and miR-155 in HGPIN. The variation in miR-155 expression and the percentage of the macrophage may be caused by epigenetic changes. Therefore, further research is needed to validate these results and understand the possibility of being a biomarker in precancerous disease of the prostate. Keywords: Prostatic Intraepithelial Neoplasia, miR-155, Macrophage
Risk factors of death among children hospitalized with social insurance (BPJS): a cross sectional study using hospital claim data Opitasari, Cicih; Avrina, Rossa; Anggraini, Anggita Bunga
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.3951

Abstract

Latar belakang: Angka kematian di rumah sakit merupakan salah satu indikator yang digunakan untuk mengukur kinerja dan kualitas pelayanan. Tujuan penelitian ini untuk menganalisis faktor risiko kematian pada anak yang dirawat dengan BPJS kesehatan di satu rumah sakit di Jakarta. Metode: Penelitian potong lintang pada satu rumah sakit pemerintah di Jakarta. Sampel menggunakan semua data klaim pasien BPJS selama periode Januari - Desember 2017. Semua pasien BPJS berusia di bawah 18 tahun yang dirawat dimasukkan dalam analisis. Regresi logistik digunakan untuk menganalisis faktor risiko kematian anak. Hasil: Dari total 18,941 jumlah pasien BPJS yang dirawat, sebanyak 3689 data anak yang dianalisis. Proporsi angka kematian anak selama satu tahun sebesar 7,3%. Kasus dengan tingkat keparahan derajat II memiliki risiko kematian 11,51 kali lipat [rasio odds suaian (ORa) = 11,51; IK=7,45-17,78; P = 0,000] dibandingkan tingkat keparahan penyakit derajat I, sedangkan kasus dengan tingkat keparahan derajat III beresiko terhadap kematian 33,97 kali lipat (ORa = 33,97;IK=19,93-57,91; P = 0,000). Selain itu, anak yang memiliki indikasi dirawat di ICU meningkatkan risiko kematian 14,21 kali lipat (ORa = 14,21; IK=9,15-22,08; P= 0,000) dibandingkan yang tidak ada indikasi ICU. Kondisi tertentu yang timbul pada periode perinatal meningkatkan risiko kematian anak 7,65 kali lipat (ORa = 7,65 ; IK=1,81-32,35;P = 0,006) dibandingkan penyakit pada sistem muskuloskeletal dan jaringan ikat. Kesimpulan: Tingkat keparahan penyakit, indikasi ICU dan kondisi tertentu yang timbul pada periode perinatal adalah faktor risiko kematian anak yang paling sering di rumah sakit Kata kunci: Faktor risiko, kematian, anak, BPJS Abstract Background: Hospital death rate is one of the indicators used to measure hospital performance and quality of care, especially the overall hospital death rate. This study aims to analyze the risk factors of death among children hospitalized with social insurance (BPJS) in one hospital in Jakarta. Methods: This was a cross-sectional study conducted in one government hospital in Jakarta. The sample was all individual claim data of BPJS patients who were hospitalized during the period of January to December 2017. All BPJS patients aged below 18 years admitted into the pediatric wards were included in the analysis. The logistic regression was used to analyze the risks of children death Results: A total of 18.941 BPJS inpatients in the hospital was identified, out of them 3689 met the inclusion criteria. The proportion of death in children during one year was 7.3%. Illness severity level II had 11.51-fold [adjusted odds ratio (ORa)=11.51;CI=7.45-17.78; P=0.000]] meanwhile severity level III had 33.97-fold higher risk of children death (ORa=33.97; CI=19.93-57.91;P=0.000) compared to children with severity level I. Children who had ICU indicator increase risk of children death at 14.21 -fold (ORa=14.21;IK=9.15-22.08;P= 0.000) compared to those who did not have. Furthermore the risk of children death in certain conditions originating in the perinatal period increases by 7.65–fold (ORa=7.65 ;IK=1.81-32.35;P=0.006) compared to diseases of the musculoskeletal system and connective tissue. Conclusion: Illness severity level, ICU indicator and diseases in certain conditions originating in the perinatal period are the most common risk factors for children death in the hospital Keywords: Risk factors, death, children, BPJS
Comparison of DNA extraction methods for molecular identification of pathogenic Leptospira in the urine samples Handayani, Farida Dwi; Wijayaningsih, Rahmi Ayu; Gasem, Muhammad Hussein; Wibawa, Tri
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.3749

Abstract

Latar belakang: Leptospirosis merupakan zoonosis penting di dunia, yang masih sering terjadi salah diagnosis. Deteksi laboratorium Leptospira menjadi tantangan karena bakterimea cukup singkat untuk dideteksi molekuler, namun antibodi juga muncul sangat lambat. Urine dapat menjadi sampel alternatif untuk deteksi PCR pada leptospirosis. Pengerjaan PCR membutuhkan DNA berkualitas dan andal, dan diperoleh dari metode ekstraksi DNA yang baik. Penelitian bertujuan untuk mengetahui metode ekstraksi DNA Leptospira terbaik untuk sampel urin, serta mengevaluasi pengaruh waktu penyimpanan dan suhu terhadap kestabilan DNA. Metode: Penelitian ini menggunakan tiga metode isolasi DNA yang berbeda; berbasis silika dengan spin kolom, kromatografi spin column menggunakan resin sebagai matriks pemisah, dan metode larutan dengan guanidine isothiocyanate. Hasil ekstraksi diperiksa konsentrasi dan kemurniannya. Gen SecY pada Leptospira dideteksi dengan PCR real-time. Pengaruh suhu dan lama penyimpanan DNA juga dilihat. Hasil: Hasil isolasi DNA menggunakan resin menunjukkan konsentrasi tertinggi (7,94 + 2,11 μg / mL) dan jumlah salinan amplifikasi DNA Leptospira tertinggi (50167,92 + 1,19). Suhu penyimpanan pada suhu 4°C, -20°C, dan -80°C dan umur simpan 91 hari tidak berpengaruh terhadap kualitas dan kuantitas DNA Leptospira hasil isolasi spike urin. Kesimpulan: Isolasi DNA menggunakan spin column chromatography dengan resin sebagai matriks separasi memiliki kualitas dan kuantitas terbaik berdasarkan kemurnian dan konsentrasi DNA serta jumlah gen SecY yang teramplifikasi. Kata kunci: Leptospira, Leptospirosis, ekstraksi DNA, sampel urin, penyimpanan sampel. Abstract Background: Leptospirosis is a worldwide zoonotic disease, which is still often misdiagnosed. Laboratory detection of Leptospira is challenging since the bacteraemia is quite short for molecular detection, however, the rise of the antibody is late to post the infection. Urine can be a potential alternative sample for PCR detection in leptospirosis. The PCR method requires a reliable DNA template, which is obtained from good DNA extracting methods. The study aimed to determine the best method of extraction Leptospira DNA from the urine sample, as well as evaluating the effect of time storage and temperature for its DNA stability. Methods: This study was utilizing three different DNA isolation methods; silica based with spin column, spin column chromatography using resin as separation matrix, and solution method with guanidine isothiocyanate. The yields were examined for its concentration and purity. Leptospira’s SecY gene was detected with realtime PCR. The influences of storage temperature and the life time of the DNA were also studied. Results: The yield of DNA isolation using resin showed the highest concentration (7.94+2.11 μg/mL) and highest Leptospira DNA amplification copy number (50167.92+1.19). Storage temperature at 4°C, -20°C, and -80°C and life time of 91 days did not have any effect on the quality and quatnity of Leptospira DNA isolated from spiked urine. Conclusions: DNA isolation using spin column chromatography with resin as separation matrix has the best quality and quantity based on the purity and concentration of DNA and the higher number of amplified SecY gene. Keywords: Leptospira, Leptospirosis, DNA extraction, urine sample, sample storage
In silico analysis of V48A dihydropteroate synthase mutation to dapsone on Mycobacterium leprae from Papua Maladan, Yustinus; Krismawati, Hana; Hutapea, Hotma Martogi Laurensia; Oktavian, Antonius
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.3744

Abstract

Latar belakang: Lepra merupakan penyakit yang disebabkan oleh Mycobacterium leprae. Resistensi obat merupakan salah satu tantangan dalam pemberantasan kusta khususnya di Papua. Adanya mutasi pada gen folP1 penyandi dihydropteroate synthase (DHPS) merupakan dasar untuk deteksi molekuler resistensi dapson pada penyakit lepra. Tujuan penelitian ini adalah mendeteksi mutasi pada gen folP1 Mycobacterium leprae dari Papua, Indonesia dan menganalisis pengaruh mutasi tersebut terhadap dapson dengan metode in silico. Metode: Identifikasi mutasi pada gen folp1 M. leprae dilakukan melalui proses Basic Local Alignment Search Tool (BLAST) di gene bank. Analisis efek mutasi dengan menggunakan server Have (y) Our Protein Explained (HOPE). Prediksi binding pocket menggunakan Computed Atlas of Surface Topography of proteins (CASTp). Homologi modeling struktur 3D DHPS menggunakan server Iterative Threading ASSEmbly Refinement (I TASSER). Analisis docking dengan menggunakan AutoDock Vina yang terintegrasi dengan aplikasi Python Prescription (PyRx). Hasil: Hasil sekuensing menunjukkan adanya variasi dalam gen folP1 M. leprae yaitu perubahan dari Timin (T) menjadi Sitosin (C) pada nukleotida 143. Residu yang bermutasi (V48A) terletak pada domain yang penting untuk aktivitas protein dan kontak dengan residu di domain lain. Ada kemungkinan bahwa interaksi ini penting untuk fungsi protein secara benar. Mutan V48A tidak banyak mempengaruhi stabilitas dari dihydropteroate synthase M. leprae. Kesimpulan: Berdasarkan analisis molecular docking, mutasi V48A tidak mempengaruhi binding affinity dapson terhadap dihydropteroate synthase M. leprae. Hasil ini menunjukkan mutan V48A kemungkinan tetaprentan terhadap dapson. Dengan demikian perlu dilakukan uji in vivo untuk mengkofirmasi efek mutasi V48A. Kata kunci: Mycobacterium leprae, folP1 gene, dihydropteroate synthase, dapson Abstract Background: Leprosy is a disease caused by Mycobacterium leprae. Drug resistance is one of the challenges in leprosy elimination especially in Papua. The presence of mutations in folP1 gene that encode dihydropteroate synthase (DHPS) was considered as the exclusive basis for molecular detection of dapsone resistance in leprosy. The objective of this study was to detect mutations in the folP1 gene of Mycobacterium leprae from Papua, Indonesia and to analyze the effect of these mutations on dapsone using the in-silico method. Methods: Identification of mutations in the folp1 M. leprae gene is carried out through the Basic Local Alignment Search Tool (BLAST) process in the gene bank. The analysis of the effects of mutations using the Have (y)Our Protein Explained (HOPE) server. Bindings pocket prediction is done using the Computed Atlas of Surface Topography of proteins (CASTp). Homology modeling 3D structure of DHPS using the Iterative Threading ASSEmbly Refinement (I-TASSER) server. Docking analysis was performed using AutoDock Vina which is integrated with the Python Prescription (PyRx) application. Results: The sequencing results showed a variation in the folP1 M. leprae gene, namely a change from thymine (T) to cytosine (C) in nucleotide 143. The mutated residue (V48A) is in a domain that is essential for the activity of the protein and in contact with residues in another domain. It is possible that this interaction is important for the correct function of the protein. V48A mutants did not significantly affect the stability of DHPS M. leprae. Conclusion: Based on molecular docking analysis, this mutation does not affect binding affinity dapsone against M. leprae dihydropteroate synthase. These results indicate that the V48A mutant is likely to remain susceptible to dapsone. Thus, it is necessary to do an in vivo test to confirm the effect of the V48A mutation. Keywords: Mycobacterium leprae, folP1 gene, dihydropteroate synthase, dapsone
Front Matter HSJi Volume 11 no.2 2019 author, hsji
Health Science Journal of Indonesia Vol 10 No 2 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

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Front Matter HSJi Volume 11 no.2 2019
Front Matter HSJi Volume 11 no.1 2020 author, hsji
Health Science Journal of Indonesia Vol 11 No 1 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

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Front Matter HSJi Volume 11 no.2 2019